Detection of pneumocystis jirovecii dihydropteroate synthase polymorphisms in patients with Pneumocystis pneumonia

M. C. Costa, J. Gaspar, K. Mansinho, F. Esteves, F. Antunes, O. Matos

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

In the present study, in order to improve the detection of Pneumocystis jirovecii dihydropteroate synthase (DHPS) mutations in pulmonary specimens of HIV-infected patients with P. jirovecii pneumonia, we evaluated a microfiltration procedure for the removal of human cell contamination and a nested-PCR method, for amplification in specimens with low parasite load. In the studied population, PCR amplification of the DHPS gene was more successful in unfiltered than in filtered specimens, with both touchdown-PCR and nested-PCR procedures (p < 0.05 and p < 0.001, respectively), but the amount of host DNA in the samples analysed seems to be inversely related with the successful PCR parasite detection. Amplification of P. jirovecii DHPS gene with nested-PCR was achieved in 77.5% of the specimens studied, demonstrating that this is a useful method for the identification of mutations in pulmonary specimens, including samples with low parasite loads, and will facilitate the evaluation of the relationship between the P. jirovecii DHPS polymorphisms and clinical resistance to sulfa drugs.

Original languageEnglish
Pages (from-to)766-771
Number of pages6
JournalScandinavian Journal Of Infectious Diseases
Volume37
Issue number10
DOIs
Publication statusPublished - 21 Nov 2005

Fingerprint

Dive into the research topics of 'Detection of pneumocystis jirovecii dihydropteroate synthase polymorphisms in patients with Pneumocystis pneumonia'. Together they form a unique fingerprint.

Cite this