Detection of nitric oxide by electron paramagnetic resonance spectroscopy: Spin-trapping with iron-dithiocarbamates

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Electron paramagnetic resonance (EPR) spectroscopy is the ideal methodology to identify radicals (detection and characterization of molecular structure) and to study their kinetics, in both simple and complex biological systems. The very low concentration and short life-time of NO and of many other radicals do not favor its direct detection and spin-traps are needed to produce a new and persistent radical that can be subsequently detected by EPR spectroscopy. In this chapter, we present the basic concepts of EPR spectroscopy and of some spin-trapping methodologies to study NO. The “strengths and weaknesses” of iron-dithiocarbamates utilization, the NO traps of choice for the authors, are thoroughly discussed and a detailed description of the method to quantify the NO formation by molybdoenzymes is provided. © Springer Science+Business Media New York 2016.
Original languageEnglish
Title of host publicationPlant Nitric Oxide
Subtitle of host publicationMethods and Protocols
EditorsKapuganti Jagadis Gupta
Place of PublicationNew York
PublisherSpringer New York
Number of pages22
ISBN (Electronic)978-1-4939-3600-7
ISBN (Print)978-1-4939-3598-7
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
PublisherSpringer New York
ISSN (Print)1064-3745


  • Aldehyde oxidoreductase
  • Electron paramagnetic resonance (EPR)
  • Iron-dithiocarbamate
  • Nitric oxide radical
  • Nitrite
  • Spin-trap
  • Xanthine oxidoreductase


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