Detection of nitric oxide by electron paramagnetic resonance spectroscopy: Spin-trapping with iron-dithiocarbamates

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Electron paramagnetic resonance (EPR) spectroscopy is the ideal methodology to identify radicals (detection and characterization of molecular structure) and to study their kinetics, in both simple and complex biological systems. The very low concentration and short life-time of NO and of many other radicals do not favor its direct detection and spin-traps are needed to produce a new and persistent radical that can be subsequently detected by EPR spectroscopy. In this chapter, we present the basic concepts of EPR spectroscopy and of some spin-trapping methodologies to study NO. The “strengths and weaknesses” of iron-dithiocarbamates utilization, the NO traps of choice for the authors, are thoroughly discussed and a detailed description of the method to quantify the NO formation by molybdoenzymes is provided. © Springer Science+Business Media New York 2016.
Original languageEnglish
Title of host publicationPlant Nitric Oxide
Subtitle of host publicationMethods and Protocols
EditorsKapuganti Jagadis Gupta
Place of PublicationNew York
PublisherSpringer New York
Pages81-102
Number of pages22
ISBN (Electronic)978-1-4939-3600-7
ISBN (Print)978-1-4939-3598-7
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
PublisherSpringer New York
Volume1424
ISSN (Print)1064-3745

Fingerprint

Spin Trapping
Electron Spin Resonance Spectroscopy
Spectrum Analysis
Nitric Oxide
Iron
Molecular Structure

Keywords

  • Aldehyde oxidoreductase
  • Electron paramagnetic resonance (EPR)
  • Iron-dithiocarbamate
  • Nitric oxide radical
  • Nitrite
  • Spin-trap
  • Xanthine oxidoreductase

Cite this

Maia, L. B., & Moura, J. J. G. (2016). Detection of nitric oxide by electron paramagnetic resonance spectroscopy: Spin-trapping with iron-dithiocarbamates. In K. J. Gupta (Ed.), Plant Nitric Oxide: Methods and Protocols (pp. 81-102). (Methods in Molecular Biology; Vol. 1424). New York: Springer New York. https://doi.org/10.1007/978-1-4939-3600-7_8
Maia, L.B. ; Moura, J.J.G. / Detection of nitric oxide by electron paramagnetic resonance spectroscopy : Spin-trapping with iron-dithiocarbamates. Plant Nitric Oxide: Methods and Protocols. editor / Kapuganti Jagadis Gupta. New York : Springer New York, 2016. pp. 81-102 (Methods in Molecular Biology).
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Maia, LB & Moura, JJG 2016, Detection of nitric oxide by electron paramagnetic resonance spectroscopy: Spin-trapping with iron-dithiocarbamates. in KJ Gupta (ed.), Plant Nitric Oxide: Methods and Protocols. Methods in Molecular Biology, vol. 1424, Springer New York, New York, pp. 81-102. https://doi.org/10.1007/978-1-4939-3600-7_8

Detection of nitric oxide by electron paramagnetic resonance spectroscopy : Spin-trapping with iron-dithiocarbamates. / Maia, L.B.; Moura, J.J.G.

Plant Nitric Oxide: Methods and Protocols. ed. / Kapuganti Jagadis Gupta. New York : Springer New York, 2016. p. 81-102 (Methods in Molecular Biology; Vol. 1424).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Detection of nitric oxide by electron paramagnetic resonance spectroscopy

T2 - Spin-trapping with iron-dithiocarbamates

AU - Maia, L.B.

AU - Moura, J.J.G.

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PY - 2016

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N2 - Electron paramagnetic resonance (EPR) spectroscopy is the ideal methodology to identify radicals (detection and characterization of molecular structure) and to study their kinetics, in both simple and complex biological systems. The very low concentration and short life-time of NO and of many other radicals do not favor its direct detection and spin-traps are needed to produce a new and persistent radical that can be subsequently detected by EPR spectroscopy. In this chapter, we present the basic concepts of EPR spectroscopy and of some spin-trapping methodologies to study NO. The “strengths and weaknesses” of iron-dithiocarbamates utilization, the NO traps of choice for the authors, are thoroughly discussed and a detailed description of the method to quantify the NO formation by molybdoenzymes is provided. © Springer Science+Business Media New York 2016.

AB - Electron paramagnetic resonance (EPR) spectroscopy is the ideal methodology to identify radicals (detection and characterization of molecular structure) and to study their kinetics, in both simple and complex biological systems. The very low concentration and short life-time of NO and of many other radicals do not favor its direct detection and spin-traps are needed to produce a new and persistent radical that can be subsequently detected by EPR spectroscopy. In this chapter, we present the basic concepts of EPR spectroscopy and of some spin-trapping methodologies to study NO. The “strengths and weaknesses” of iron-dithiocarbamates utilization, the NO traps of choice for the authors, are thoroughly discussed and a detailed description of the method to quantify the NO formation by molybdoenzymes is provided. © Springer Science+Business Media New York 2016.

KW - Aldehyde oxidoreductase

KW - Electron paramagnetic resonance (EPR)

KW - Iron-dithiocarbamate

KW - Nitric oxide radical

KW - Nitrite

KW - Spin-trap

KW - Xanthine oxidoreductase

U2 - 10.1007/978-1-4939-3600-7_8

DO - 10.1007/978-1-4939-3600-7_8

M3 - Chapter

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Maia LB, Moura JJG. Detection of nitric oxide by electron paramagnetic resonance spectroscopy: Spin-trapping with iron-dithiocarbamates. In Gupta KJ, editor, Plant Nitric Oxide: Methods and Protocols. New York: Springer New York. 2016. p. 81-102. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3600-7_8