Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®: a multicenter study

Silvana Spindola de Miranda, Isabela Neves de Almeida, Maria de Fátima Filardi Oliveira Mansur, Lida Jouca de Assis Figueredo, Wânia da Silva Carvalho, João Paulo Amaral Hadaad, Jaciara de Lourdes do Carmo Guimarães Diniz, Andrea von Groll, Pedro Almeida da Silva, Maria Luiza Lopes, Marcelo Cordeiro dos Santos, Alexandra Brito, Fernanda Carvalho de Queiroz Mello, Thiago da Silva Santos Malaquias, Julio Croda, Juliana Maira Watanabe Pinhata, Rosângela Siqueira de Oliveira, Erica Chimara, Maria Lúcia Rossetti, Maria Laura HalonMaria Cristina Lourenço, Reginalda Ferreira de Melo Medeiros, Fátima Cristina Onofre Fandinho Montes, Diana Machado, Miguel Viveiros, Afrânio Lineu Kritski

Research output: Contribution to journalArticlepeer-review

Abstract

(1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.

Original languageEnglish
Pages (from-to)e20190179-e20190188
Number of pages9
JournalBrazilian Archives Of Biology And Technology
Volume63
DOIs
Publication statusPublished - 2020

Keywords

  • MDR
  • Nitrate reductase
  • Technology
  • Tuberculosis

UN Sustainable Development Goals (SDGs)

  • SDG 3 - Good Health and Well-Being

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