TY - JOUR
T1 - Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®
T2 - a multicenter study
AU - de Miranda, Silvana Spindola
AU - de Almeida, Isabela Neves
AU - de Fátima Filardi Oliveira Mansur, Maria
AU - de Assis Figueredo, Lida Jouca
AU - da Silva Carvalho, Wânia
AU - Hadaad, João Paulo Amaral
AU - de Lourdes do Carmo Guimarães Diniz, Jaciara
AU - von Groll, Andrea
AU - da Silva, Pedro Almeida
AU - Lopes, Maria Luiza
AU - dos Santos, Marcelo Cordeiro
AU - Brito, Alexandra
AU - de Queiroz Mello, Fernanda Carvalho
AU - da Silva Santos Malaquias, Thiago
AU - Croda, Julio
AU - Pinhata, Juliana Maira Watanabe
AU - de Oliveira, Rosângela Siqueira
AU - Chimara, Erica
AU - Rossetti, Maria Lúcia
AU - Halon, Maria Laura
AU - Lourenço, Maria Cristina
AU - de Melo Medeiros, Reginalda Ferreira
AU - Montes, Fátima Cristina Onofre Fandinho
AU - Machado, Diana
AU - Viveiros, Miguel
AU - Kritski, Afrânio Lineu
PY - 2020
Y1 - 2020
N2 - (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.
AB - (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.
KW - MDR
KW - Nitrate reductase
KW - Technology
KW - Tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=85089808552&partnerID=8YFLogxK
UR - https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132020000100308&lng=en&nrm=iso&tlng=en
U2 - 10.1590/1678-4324-2020190179
DO - 10.1590/1678-4324-2020190179
M3 - Article
AN - SCOPUS:85089808552
SN - 1516-8913
VL - 63
SP - e20190179-e20190188
JO - Brazilian Archives Of Biology And Technology
JF - Brazilian Archives Of Biology And Technology
ER -
