Detection of cardiac biomarker proteins using a disposable based on a molecularly imprinted polymer grafted onto graphite

Felismina T. C. Moreira, Sanjiv Sharma, Rosa A. F. Dutra, João Paulo da Costa de Noronha, Anthony E. G. Cass, M. Goreti F Sales

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A low-cost disposable was developed for rapid detection of the protein biomarker myoglobin (Myo) as a model analyte. A screen printed electrode was modified with a molecularly imprinted material grafted on a graphite support and incorporated in a matrix composed of poly(vinyl chloride) and the plasticizer o-nitrophenyloctyl ether. The protein-imprinted material (PIM) was produced by growing a reticulated polymer around a protein template. This is followed by radical polymerization of 4-styrenesulfonic acid, 2-aminoethyl methacrylate hydrochloride, and ethylene glycol dimethacrylate. The polymeric layer was then covalently bound to the graphitic support, and Myo was added during the imprinting stage to act as a template. Non-imprinted control materials (CM) were also prepared by omitting the Myo template. Morphological and structural analysis of PIM and CM byFTIR, Raman, and SEM/EDC microscopies confirmed the modification of the graphite support. The analytical performance of the SPE was assessed by square wave voltammetry. The average limit of detection is 0.79μg of Myo per mL, and the slope is −0.193 ± 0.006μA per decade. The SPE-CM cannot detect such low levels of Myo but gives a linear response at above 7.2μg · mL−1, with a slope of −0.719 ± 0.02μA per decade. Interference studies with hemoglobin, bovine serum albumin, creatinine, and sodium chloride demonstrated good selectivity for Myo. The method was successfully applied to the determination of Myo urine and is conceived to be a promising tool for screening Myo in point-of-care patients with ischemia.
Original languageEnglish
Pages (from-to)975-983
JournalMicrochimica Acta
Volume182
Issue number5-6
DOIs
Publication statusPublished - Apr 2015

Keywords

  • Biosensor
  • Cardiac biomarker
  • Myoglobin
  • Potentiometry
  • Protein imprinting
  • Screen-printed electrodes

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