Detection of atovaquone and MalaroneTM resistance conferring mutations in Plasmodium falciparum cytochrome b gene (cytb)

J. P. Gil; F Nogueira; J. Strömberg-Nörklit; J. Lindberg; M. Carrolo; C. Casimiro; D Lopes; Ana Paula Arez; Pedro Vitor Lemos Cravo; Virgilio Estólio do Rosário

doi:10.1016/s0890-8508(03)00006-9

Detection of atovaquone and MalaroneTM resistance conferring mutations in Plasmodium falciparum cytochrome b gene (cytb)

J. P. Gil, F Nogueira, J. Strömberg-Nörklit, J. Lindberg, M. Carrolo, C. Casimiro, D Lopes, Ana Paula Arez, Pedro Vitor Lemos Cravo, Virgilio Estólio do Rosário

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12 Citations (Scopus)

Abstract

Clinical treatment failures of the hydroxynaphthoquinone atovaquone or its combination with proguanil (Malarone) in Plasmodium falciparum malaria has been recently documented. These events have been associated to single nucleotide polymorphisms (SNPs) in the parasite cytochrome b gene (cytb). In this report we describe a set of nest PCR-RFLP methods developed for the fast detection of all known cytb mutations associated to resistance to these drugs. The methods were successfully applied for the analysis of phenol-chloroform extracted DNA samples from patients not cured by Malarone, and from an established parasite clone. Further, the protocol for the detection of the A803C mutation was applied to 164 DNA field samples extracted through crude methanol-based protocols, originated from several malaria settings. The PCR-RFLP methods here presented can be used as a valuable for the clinical detection and study of Malarone and atovaquone P. falciparum resistance.

Original language	English
Pages (from-to)	85-89
Number of pages	5
Journal	Molecular and Cellular Probes
Volume	17
Issue number	2-3
DOIs	https://doi.org/10.1016/s0890-8508(03)00006-9
Publication status	Published - Apr 2003

Keywords

Plasmodium falciparum
Resistance
Cytochrome b
Atovaquone
Malarone
PCR-RFLP

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/s0890-8508(03)00006-9

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Gil, J. P., Nogueira, F., Strömberg-Nörklit, J., Lindberg, J., Carrolo, M., Casimiro, C., Lopes, D., Arez, A. P., Cravo, P. V. L., & Rosário, V. E. D. (2003). Detection of atovaquone and MalaroneTM resistance conferring mutations in Plasmodium falciparum cytochrome b gene (cytb). Molecular and Cellular Probes, 17(2-3), 85-89. https://doi.org/10.1016/s0890-8508(03)00006-9

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title = "Detection of atovaquone and MalaroneTM resistance conferring mutations in Plasmodium falciparum cytochrome b gene (cytb)",

abstract = "Clinical treatment failures of the hydroxynaphthoquinone atovaquone or its combination with proguanil (Malarone) in Plasmodium falciparum malaria has been recently documented. These events have been associated to single nucleotide polymorphisms (SNPs) in the parasite cytochrome b gene (cytb). In this report we describe a set of nest PCR-RFLP methods developed for the fast detection of all known cytb mutations associated to resistance to these drugs. The methods were successfully applied for the analysis of phenol-chloroform extracted DNA samples from patients not cured by Malarone, and from an established parasite clone. Further, the protocol for the detection of the A803C mutation was applied to 164 DNA field samples extracted through crude methanol-based protocols, originated from several malaria settings. The PCR-RFLP methods here presented can be used as a valuable for the clinical detection and study of Malarone and atovaquone P. falciparum resistance.",

keywords = "Plasmodium falciparum, Resistance, Cytochrome b, Atovaquone, Malarone, PCR-RFLP",

author = "Gil, {J. P.} and F Nogueira and J. Str{\"o}mberg-N{\"o}rklit and J. Lindberg and M. Carrolo and C. Casimiro and D Lopes and Arez, {Ana Paula} and Cravo, {Pedro Vitor Lemos} and Ros{\'a}rio, {Virgilio Est{\'o}lio do}",

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doi = "10.1016/s0890-8508(03)00006-9",

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T1 - Detection of atovaquone and MalaroneTM resistance conferring mutations in Plasmodium falciparum cytochrome b gene (cytb)

AU - Gil, J. P.

AU - Nogueira, F

AU - Strömberg-Nörklit, J.

AU - Lindberg, J.

AU - Carrolo, M.

AU - Casimiro, C.

AU - Lopes, D

AU - Arez, Ana Paula

AU - Cravo, Pedro Vitor Lemos

AU - Rosário, Virgilio Estólio do

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N2 - Clinical treatment failures of the hydroxynaphthoquinone atovaquone or its combination with proguanil (Malarone) in Plasmodium falciparum malaria has been recently documented. These events have been associated to single nucleotide polymorphisms (SNPs) in the parasite cytochrome b gene (cytb). In this report we describe a set of nest PCR-RFLP methods developed for the fast detection of all known cytb mutations associated to resistance to these drugs. The methods were successfully applied for the analysis of phenol-chloroform extracted DNA samples from patients not cured by Malarone, and from an established parasite clone. Further, the protocol for the detection of the A803C mutation was applied to 164 DNA field samples extracted through crude methanol-based protocols, originated from several malaria settings. The PCR-RFLP methods here presented can be used as a valuable for the clinical detection and study of Malarone and atovaquone P. falciparum resistance.

AB - Clinical treatment failures of the hydroxynaphthoquinone atovaquone or its combination with proguanil (Malarone) in Plasmodium falciparum malaria has been recently documented. These events have been associated to single nucleotide polymorphisms (SNPs) in the parasite cytochrome b gene (cytb). In this report we describe a set of nest PCR-RFLP methods developed for the fast detection of all known cytb mutations associated to resistance to these drugs. The methods were successfully applied for the analysis of phenol-chloroform extracted DNA samples from patients not cured by Malarone, and from an established parasite clone. Further, the protocol for the detection of the A803C mutation was applied to 164 DNA field samples extracted through crude methanol-based protocols, originated from several malaria settings. The PCR-RFLP methods here presented can be used as a valuable for the clinical detection and study of Malarone and atovaquone P. falciparum resistance.

KW - Plasmodium falciparum

KW - Resistance

KW - Cytochrome b

KW - Atovaquone

KW - Malarone

KW - PCR-RFLP

UR - https://www.sciencedirect.com/science/article/abs/pii/S0890850803000069?via%3Dihub

U2 - 10.1016/s0890-8508(03)00006-9

DO - 10.1016/s0890-8508(03)00006-9

M3 - Article

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SN - 0890-8508

VL - 17

SP - 85

EP - 89

JO - Molecular and Cellular Probes

JF - Molecular and Cellular Probes

IS - 2-3

ER -

Detection of atovaquone and MalaroneTM resistance conferring mutations in Plasmodium falciparum cytochrome b gene (cytb)

Abstract

Keywords

UN SDGs

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