Designed affinity ligands to capture human serum albumin

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7 Citations (Scopus)

Abstract

Human serum albumin (HSA) in an important therapeutic agent and disease biomarker, with an increasing market demand. By proteins and drugs that bind to HSA as inspiration, a combinatorial library of 64 triazine-based ligands was rationally designed and screened for HSA binding at physiological conditions. Two triazine-based lead ligands (A3A2 and A6A5), presenting more than 50% HSA bound and high enrichment factors, were selected for further studies. Binding and elution conditions for HSA purification from human plasma were optimized for both ligands. The A6A5 adsorbent yielded a purified HSA sample with 98% purity at 100% recovery yield under mild binding and elution conditions.

Original languageEnglish
Pages (from-to)88-97
JournalJournal Of Chromatography A
Volume1583
DOIs
Publication statusPublished - 4 Jan 2019

Keywords

  • Affinity ligands
  • Combinatorial chemistry
  • Human serum albumin
  • Protein purification
  • Synthetic ligands

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