Designed affinity ligands to capture human serum albumin

Research output: Contribution to journalArticle

Abstract

Human serum albumin (HSA) in an important therapeutic agent and disease biomarker, with an increasing market demand. By proteins and drugs that bind to HSA as inspiration, a combinatorial library of 64 triazine-based ligands was rationally designed and screened for HSA binding at physiological conditions. Two triazine-based lead ligands (A3A2 and A6A5), presenting more than 50% HSA bound and high enrichment factors, were selected for further studies. Binding and elution conditions for HSA purification from human plasma were optimized for both ligands. The A6A5 adsorbent yielded a purified HSA sample with 98% purity at 100% recovery yield under mild binding and elution conditions.

Original languageEnglish
Pages (from-to)88-97
JournalJournal Of Chromatography A
Volume1583
DOIs
Publication statusPublished - 4 Jan 2019

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Serum Albumin
Ligands
Triazines
Plasma (human)
Biomarkers
Adsorbents
Purification
Recovery
Pharmaceutical Preparations
Proteins

Keywords

  • Affinity ligands
  • Combinatorial chemistry
  • Human serum albumin
  • Protein purification
  • Synthetic ligands

Cite this

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title = "Designed affinity ligands to capture human serum albumin",
abstract = "Human serum albumin (HSA) in an important therapeutic agent and disease biomarker, with an increasing market demand. By proteins and drugs that bind to HSA as inspiration, a combinatorial library of 64 triazine-based ligands was rationally designed and screened for HSA binding at physiological conditions. Two triazine-based lead ligands (A3A2 and A6A5), presenting more than 50{\%} HSA bound and high enrichment factors, were selected for further studies. Binding and elution conditions for HSA purification from human plasma were optimized for both ligands. The A6A5 adsorbent yielded a purified HSA sample with 98{\%} purity at 100{\%} recovery yield under mild binding and elution conditions.",
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author = "{dos Santos}, Raquel and Carina Figueiredo and Viecinski, {Aline Canani} and Pina, {Ana Sofia} and Barbosa, {Arm{\'e}nio J. M.} and Roque, {A. Cec{\'i}lia A.}",
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AU - Figueiredo, Carina

AU - Viecinski, Aline Canani

AU - Pina, Ana Sofia

AU - Barbosa, Arménio J. M.

AU - Roque, A. Cecília A.

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AB - Human serum albumin (HSA) in an important therapeutic agent and disease biomarker, with an increasing market demand. By proteins and drugs that bind to HSA as inspiration, a combinatorial library of 64 triazine-based ligands was rationally designed and screened for HSA binding at physiological conditions. Two triazine-based lead ligands (A3A2 and A6A5), presenting more than 50% HSA bound and high enrichment factors, were selected for further studies. Binding and elution conditions for HSA purification from human plasma were optimized for both ligands. The A6A5 adsorbent yielded a purified HSA sample with 98% purity at 100% recovery yield under mild binding and elution conditions.

KW - Affinity ligands

KW - Combinatorial chemistry

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KW - Protein purification

KW - Synthetic ligands

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