Design of pathway-level bioprocess monitoring and control strategies supported by metabolic networks

Inês A. Isidro, Ana R. Ferreira, João J. Clemente, António E. Cunha, João M.L. Dias, Rui Oliveira

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Citation (Scopus)


In this chapter we explore the basic tools for the design of bioprocess monitoring, optimization, and control algorithms that incorporate a priori knowledge of metabolic networks. The main advantage is that this ultimately enables the targeting of intracellular control variables such as metabolic reactions or metabolic pathways directly linked with productivity and product quality. We analyze in particular design methods that target elementary modes of metabolic networks. The topics covered include the analysis of the structure of metabolic networks, computation and reduction of elementary modes, measurement methods for the envirome, envirome-guided metabolic reconstruction, and macroscopic dynamic modeling and control. These topics are illustrated with applications to a cultivation process of a recombinant Pichia pastoris X33 strain expressing a single-chain antibody fragment (scFv).

Original languageEnglish
Title of host publicationMeasurement, Monitoring, Modelling and Control of Bioprocesses
EditorsCarl-Fredrik Mandenius, Nigel Titchener-Hooker
Number of pages23
Publication statusPublished - 30 Aug 2013

Publication series

NameAdvances in Biochemical Engineering/Biotechnology
ISSN (Print)0724-6145


  • Bioprocess control
  • Dynamic modeling
  • Elementary modes
  • Envirome measurement
  • Metabolic networks


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