Design of oligoaziridine-PEG coatings for efficient nanogold cellular biotagging

A. Sofia Silva, Vasco D. B. Bonifacio, Vivek P. Raje, Paula Cristina de Sério Branco, Paulo F. B. Machado, Ilidio J. Correia, Ana Isabel Nobre Martins Aguiar de Oliveira Ricardo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Gold nanoparticles (AuNPs) are the most investigated nanomaterials for theragnosis applications. In a research field where live cell assays, as well as the tracking of nanomaterials into a cell's environment, are of extremely importance, water-soluble AuNPs have been intensively studied to overcome the toxic effects exerted by coatings. Unfortunately, AuNPs fluorescent tagging often fails due to self-quenching and a careful design must be carried out to maintain optoelectronic properties and biocompatibility. In this work, the synthesis of fluorescent gold nanoprobes, able to enter the cell's environment (biotags) and target the cell nucleus, was designed and the particles tracked by confocal laser scanning microscopy. The coating of AuNPs with maleimide poly(ethylene glycol) and fluorescent oligoaziridine biocompatible oligomers, resulted in robust, optically active biotags that open novel insights into cancer theragnosis.

Original languageEnglish
Pages (from-to)10733-10738
Number of pages6
JournalRCS Advances
Volume5
Issue number14
DOIs
Publication statusPublished - 2015

Keywords

  • NANOPARTICLES FACILE PREPARATION
  • GOLD NANOPARTICLES
  • DRUG-DELIVERY
  • BIOMEDICAL APPLICATIONS
  • CANCER
  • DNA
  • FLUORESCENCE
  • NANOWIRES
  • THERAPY
  • SIZE

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