Demonstration of the adhesive properties of the medium-chain-length polyhydroxyalkanoate produced by Pseudomonas chlororaphis subsp. aurantiaca from glycerol

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Abstract

Pseudomonas chlororaphis subsp. aurantiaca (DSM 19603) was grown on crude glycerol from biodiesel production to produce a medium-chain-length polyhydroxyalkanoate (mcl-PHA), composed of 3-hydroxydodecanoate (43 ± 1.8 mol%), 3-hydroxydecanoate (29 ± 3.1 mol%), 3-hydroxytetradecanoate (12 ± 0.4 mol%), 3-hydroxyoctanoate (10 ± 1.5 mol%) and 3-hydroxyhexanoate (6 ± 0.3 mol%). The biopolymer had an average molecular weight of 1.1 × 105 Da, with a polydispersity index of 1.5, and was semi-crystalline, as shown by its crystallinity index of 37 ± 0.2%. It had low melting (44 °C) and glass transition (−48 °C) temperatures, and was thermally stable up to 285 °C. The biopolymer films were elastic and translucid, were hydrophobic and presented relatively high permeability to oxygen and carbon dioxide. The films demonstrated to have good adhesion properties towards porcine skin and human skin. The tension (61.1 ± 20.6 kPa) and shear (12.7 ± 2.14 kPa) bond strength of the mcl-PHA for porcine skin suggest its potential as a biomaterial for the development of novel natural adhesives for wound closure or wound dressings.

Original languageEnglish
Pages (from-to)1144-1151
JournalInternational Journal of Biological Macromolecules
Volume122
DOIs
Publication statusPublished - 1 Feb 2019

Fingerprint

Polyhydroxyalkanoates
Glycerol
Chain length
Adhesives
Skin
Biopolymers
Demonstrations
Swine
Biofuels
Wounds and Injuries
Polydispersity
Biocompatible Materials
Bandages
Biodiesel
Biomaterials
Carbon Dioxide
Freezing
Glass
Glass transition
Permeability

Keywords

  • Adhesion
  • Polyhydroxyalkanoates (PHA)
  • Pseudomonas chlororaphis

Cite this

@article{09613c5695f94eb9911446ad62ee5d9e,
title = "Demonstration of the adhesive properties of the medium-chain-length polyhydroxyalkanoate produced by Pseudomonas chlororaphis subsp. aurantiaca from glycerol",
abstract = "Pseudomonas chlororaphis subsp. aurantiaca (DSM 19603) was grown on crude glycerol from biodiesel production to produce a medium-chain-length polyhydroxyalkanoate (mcl-PHA), composed of 3-hydroxydodecanoate (43 ± 1.8 mol{\%}), 3-hydroxydecanoate (29 ± 3.1 mol{\%}), 3-hydroxytetradecanoate (12 ± 0.4 mol{\%}), 3-hydroxyoctanoate (10 ± 1.5 mol{\%}) and 3-hydroxyhexanoate (6 ± 0.3 mol{\%}). The biopolymer had an average molecular weight of 1.1 × 105 Da, with a polydispersity index of 1.5, and was semi-crystalline, as shown by its crystallinity index of 37 ± 0.2{\%}. It had low melting (44 °C) and glass transition (−48 °C) temperatures, and was thermally stable up to 285 °C. The biopolymer films were elastic and translucid, were hydrophobic and presented relatively high permeability to oxygen and carbon dioxide. The films demonstrated to have good adhesion properties towards porcine skin and human skin. The tension (61.1 ± 20.6 kPa) and shear (12.7 ± 2.14 kPa) bond strength of the mcl-PHA for porcine skin suggest its potential as a biomaterial for the development of novel natural adhesives for wound closure or wound dressings.",
keywords = "Adhesion, Polyhydroxyalkanoates (PHA), Pseudomonas chlororaphis",
author = "Pereira, {Jo{\~a}o R.} and Diana Ara{\'u}jo and Marques, {Ana C.} and Neves, {Lu{\'i}sa A.} and Christian Grandfils and Chantal Sevrin and Alves, {V{\'i}tor D.} and Elvira Fortunato and Reis, {Maria A. M.} and Filomena Freitas",
note = "info:eu-repo/grantAgreement/FCT/5876/147258/PT# info:eu-repo/grantAgreement/FCT/5876/147333/PT# This project was supported by the Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), the Associated Laboratory for Sustainable Chemistry-Clean Processes and Technologies - LAQV, the Centro de Investigacao de Materiais I i3N and LEAF-Linking Landscape, Environment, Agriculture and Food, which are financed by national funds from FCT/MCTES (UID/Multi/04378/2013, UlD/QUI/50006/2013, UID/CTM/50025/2013, PEst-OE/AGR/U10245/2014) and co-financed by the ERDF, under the FT2020 Partnership Agreement (POCI-01-0145-FEDER-007728, POCI-01-0145-FEDER-007265, POCI-01-0145-FEDER-007688), and exploratory projects IF/00505/2014 and IF/00589/2015 attributed within the FCT Researcher Program. Ana C. Marques acknowledges FCT-MCTES for grant BD/115173/2016.",
year = "2019",
month = "2",
day = "1",
doi = "10.1016/j.ijbiomac.2018.09.064",
language = "English",
volume = "122",
pages = "1144--1151",
journal = "International Journal of Biological Macromolecules",
issn = "0141-8130",
publisher = "Elsevier Science B.V., Inc",

}

TY - JOUR

T1 - Demonstration of the adhesive properties of the medium-chain-length polyhydroxyalkanoate produced by Pseudomonas chlororaphis subsp. aurantiaca from glycerol

AU - Pereira, João R.

AU - Araújo, Diana

AU - Marques, Ana C.

AU - Neves, Luísa A.

AU - Grandfils, Christian

AU - Sevrin, Chantal

AU - Alves, Vítor D.

AU - Fortunato, Elvira

AU - Reis, Maria A. M.

AU - Freitas, Filomena

N1 - info:eu-repo/grantAgreement/FCT/5876/147258/PT# info:eu-repo/grantAgreement/FCT/5876/147333/PT# This project was supported by the Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), the Associated Laboratory for Sustainable Chemistry-Clean Processes and Technologies - LAQV, the Centro de Investigacao de Materiais I i3N and LEAF-Linking Landscape, Environment, Agriculture and Food, which are financed by national funds from FCT/MCTES (UID/Multi/04378/2013, UlD/QUI/50006/2013, UID/CTM/50025/2013, PEst-OE/AGR/U10245/2014) and co-financed by the ERDF, under the FT2020 Partnership Agreement (POCI-01-0145-FEDER-007728, POCI-01-0145-FEDER-007265, POCI-01-0145-FEDER-007688), and exploratory projects IF/00505/2014 and IF/00589/2015 attributed within the FCT Researcher Program. Ana C. Marques acknowledges FCT-MCTES for grant BD/115173/2016.

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Pseudomonas chlororaphis subsp. aurantiaca (DSM 19603) was grown on crude glycerol from biodiesel production to produce a medium-chain-length polyhydroxyalkanoate (mcl-PHA), composed of 3-hydroxydodecanoate (43 ± 1.8 mol%), 3-hydroxydecanoate (29 ± 3.1 mol%), 3-hydroxytetradecanoate (12 ± 0.4 mol%), 3-hydroxyoctanoate (10 ± 1.5 mol%) and 3-hydroxyhexanoate (6 ± 0.3 mol%). The biopolymer had an average molecular weight of 1.1 × 105 Da, with a polydispersity index of 1.5, and was semi-crystalline, as shown by its crystallinity index of 37 ± 0.2%. It had low melting (44 °C) and glass transition (−48 °C) temperatures, and was thermally stable up to 285 °C. The biopolymer films were elastic and translucid, were hydrophobic and presented relatively high permeability to oxygen and carbon dioxide. The films demonstrated to have good adhesion properties towards porcine skin and human skin. The tension (61.1 ± 20.6 kPa) and shear (12.7 ± 2.14 kPa) bond strength of the mcl-PHA for porcine skin suggest its potential as a biomaterial for the development of novel natural adhesives for wound closure or wound dressings.

AB - Pseudomonas chlororaphis subsp. aurantiaca (DSM 19603) was grown on crude glycerol from biodiesel production to produce a medium-chain-length polyhydroxyalkanoate (mcl-PHA), composed of 3-hydroxydodecanoate (43 ± 1.8 mol%), 3-hydroxydecanoate (29 ± 3.1 mol%), 3-hydroxytetradecanoate (12 ± 0.4 mol%), 3-hydroxyoctanoate (10 ± 1.5 mol%) and 3-hydroxyhexanoate (6 ± 0.3 mol%). The biopolymer had an average molecular weight of 1.1 × 105 Da, with a polydispersity index of 1.5, and was semi-crystalline, as shown by its crystallinity index of 37 ± 0.2%. It had low melting (44 °C) and glass transition (−48 °C) temperatures, and was thermally stable up to 285 °C. The biopolymer films were elastic and translucid, were hydrophobic and presented relatively high permeability to oxygen and carbon dioxide. The films demonstrated to have good adhesion properties towards porcine skin and human skin. The tension (61.1 ± 20.6 kPa) and shear (12.7 ± 2.14 kPa) bond strength of the mcl-PHA for porcine skin suggest its potential as a biomaterial for the development of novel natural adhesives for wound closure or wound dressings.

KW - Adhesion

KW - Polyhydroxyalkanoates (PHA)

KW - Pseudomonas chlororaphis

UR - http://www.scopus.com/inward/record.url?scp=85053299465&partnerID=8YFLogxK

U2 - 10.1016/j.ijbiomac.2018.09.064

DO - 10.1016/j.ijbiomac.2018.09.064

M3 - Article

VL - 122

SP - 1144

EP - 1151

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

ER -