Demonstration of intrinsic efflux activity of Escherichia coli K-12 AG100 by an automated ethidium bromide method

Miguel Viveiros; Ana Martins; Laura Paixão; Liliana Rodrigues; Marta Martins; Isabel Couto; Eva Fähnrich; Winfried V. Kern; Leonard Amaral

doi:10.1016/j.ijantimicag.2007.12.015

Demonstration of intrinsic efflux activity of Escherichia coli K-12 AG100 by an automated ethidium bromide method

Miguel Viveiros, Ana Martins, Laura Paixão, Liliana Rodrigues, Marta Martins, Isabel Couto, Eva Fähnrich, Winfried V. Kern, Leonard Amaral

Research output: Contribution to journal › Article › peer-review

135 Citations (Scopus)

Abstract

Demonstration of efflux of ethidium bromide (EtBr) has been made for over 30 bacterial species, usually by showing enhanced efflux in multidrug-resistant strains that was then abolished by inactivating efflux pumps. Here we present a relatively simple automated method that employs EtBr as an efflux pump substrate for the demonstration of intrinsic efflux activity in Escherichia coli K-12 AG100. The method uses the Rotor-Gene 3000™ instrument for real-time fluorometric measurement of EtBr accumulation under conditions that limit energy (absence of glucose, low temperature) and of EtBr extrusion under optimum conditions. The method can be used for screening compound libraries for efflux inhibiting capacity.

Original language	English
Pages (from-to)	458-462
Number of pages	5
Journal	International Journal Of Antimicrobial Agents
Volume	Vol. 31
Issue number	n.º 5
DOIs	https://doi.org/10.1016/j.ijantimicag.2007.12.015
Publication status	Published - May 2008

Keywords

Automated method
Bacterial efflux pumps
Efflux pump inhibitors
Escherichia coli
Ethidium bromide
Fluorometry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.ijantimicag.2007.12.015

Cite this

@article{fac8841f62744975b1aaed809f007ca0,

title = "Demonstration of intrinsic efflux activity of Escherichia coli K-12 AG100 by an automated ethidium bromide method",

abstract = "Demonstration of efflux of ethidium bromide (EtBr) has been made for over 30 bacterial species, usually by showing enhanced efflux in multidrug-resistant strains that was then abolished by inactivating efflux pumps. Here we present a relatively simple automated method that employs EtBr as an efflux pump substrate for the demonstration of intrinsic efflux activity in Escherichia coli K-12 AG100. The method uses the Rotor-Gene 3000{\texttrademark} instrument for real-time fluorometric measurement of EtBr accumulation under conditions that limit energy (absence of glucose, low temperature) and of EtBr extrusion under optimum conditions. The method can be used for screening compound libraries for efflux inhibiting capacity.",

keywords = "Automated method, Bacterial efflux pumps, Efflux pump inhibitors, Escherichia coli, Ethidium bromide, Fluorometry",

author = "Miguel Viveiros and Ana Martins and Laura Paix{\~a}o and Liliana Rodrigues and Marta Martins and Isabel Couto and Eva F{\"a}hnrich and Kern, {Winfried V.} and Leonard Amaral",

year = "2008",

month = may,

doi = "10.1016/j.ijantimicag.2007.12.015",

language = "English",

volume = "Vol. 31",

pages = "458--462",

journal = "International Journal Of Antimicrobial Agents",

issn = "0924-8579",

publisher = "Elsevier",

number = "n.º 5",

}

TY - JOUR

T1 - Demonstration of intrinsic efflux activity of Escherichia coli K-12 AG100 by an automated ethidium bromide method

AU - Viveiros, Miguel

AU - Martins, Ana

AU - Paixão, Laura

AU - Rodrigues, Liliana

AU - Martins, Marta

AU - Couto, Isabel

AU - Fähnrich, Eva

AU - Kern, Winfried V.

AU - Amaral, Leonard

PY - 2008/5

Y1 - 2008/5

N2 - Demonstration of efflux of ethidium bromide (EtBr) has been made for over 30 bacterial species, usually by showing enhanced efflux in multidrug-resistant strains that was then abolished by inactivating efflux pumps. Here we present a relatively simple automated method that employs EtBr as an efflux pump substrate for the demonstration of intrinsic efflux activity in Escherichia coli K-12 AG100. The method uses the Rotor-Gene 3000™ instrument for real-time fluorometric measurement of EtBr accumulation under conditions that limit energy (absence of glucose, low temperature) and of EtBr extrusion under optimum conditions. The method can be used for screening compound libraries for efflux inhibiting capacity.

AB - Demonstration of efflux of ethidium bromide (EtBr) has been made for over 30 bacterial species, usually by showing enhanced efflux in multidrug-resistant strains that was then abolished by inactivating efflux pumps. Here we present a relatively simple automated method that employs EtBr as an efflux pump substrate for the demonstration of intrinsic efflux activity in Escherichia coli K-12 AG100. The method uses the Rotor-Gene 3000™ instrument for real-time fluorometric measurement of EtBr accumulation under conditions that limit energy (absence of glucose, low temperature) and of EtBr extrusion under optimum conditions. The method can be used for screening compound libraries for efflux inhibiting capacity.

KW - Automated method

KW - Bacterial efflux pumps

KW - Efflux pump inhibitors

KW - Escherichia coli

KW - Ethidium bromide

KW - Fluorometry

UR - http://www.scopus.com/inward/record.url?scp=41949109742&partnerID=8YFLogxK

UR - https://www.sciencedirect.com/science/article/pii/S0924857908000320?via%3Dihub

U2 - 10.1016/j.ijantimicag.2007.12.015

DO - 10.1016/j.ijantimicag.2007.12.015

M3 - Article

C2 - 18343640

AN - SCOPUS:41949109742

SN - 0924-8579

VL - Vol. 31

SP - 458

EP - 462

JO - International Journal Of Antimicrobial Agents

JF - International Journal Of Antimicrobial Agents

IS - n.º 5

ER -

Demonstration of intrinsic efflux activity of Escherichia coli K-12 AG100 by an automated ethidium bromide method

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this