De novo synthesis of fatty acids is regulated by FapR protein in Exiguobacterium antarcticum B7, a psychrotrophic bacterium isolated from Antarctica

Rafael A. Baraúna, Diego A. Das Graças, Catarina I.P. Nunes, Maria P.C. Schneider, Artur Silva, Marta S.P. Carepo

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Background:FapR protein from the psychrotrophic species Exiguobacterium antarcticum B7 was expressed and purified, and subsequently evaluated for its capacity to bind to the promoter regions of the fabH1-fabF and fapR-plsX-fabD-fabG operons, using electrophoretic mobility shift assay. The genes that compose these operons encode for enzymes involved in the de novo synthesis of fatty acids molecules. In Bacillus subtilis, FapR regulates the expression of these operons, and consequently has influence in the synthesis of long or short-chain fatty acids. To analyze the bacterial cold adaptation, this is an important metabolic pathway because psychrotrophic microrganisms tend to synthesize short and branched-chain unsaturated fatty acids at cold to maintain cell membrane fluidity. Results: In this work, it was observed that recombinant protein was able to bind to the promoter of the fully amplified fabH1-fabF and fapR-plsX-fabD-fabG operons. However, FapR was unable to bind to the promoter of fapR-plsX-fabD-fabG operon when synthesized only up to the protein-binding palindrome 5′-TTAGTACCAGATACTAA-3′, thus showing the importance of the entire promoter sequence for the correct protein-DNA interaction. Conclusions: Through this observation, we demonstrate that the FapR protein possibly regulates the same operons as described for other species, which emphasizes its importance to cold adaptation process of E. antarcticum B7, a psychrotrophic bacterium isolated at Antarctica.

Original languageEnglish
Article number447
Number of pages5
JournalBmc Research Notes
Volume9
Issue number1
DOIs
Publication statusPublished - 20 Sep 2016

Keywords

  • Antarctica
  • Exiguobacterium antarcticum
  • FapR
  • Psychrotrophic

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