TY - JOUR
T1 - Data descriptor
T2 - Protocols and characterization data for 2d, 3d, and slice-based tumor models from the predect project
AU - De Hoogt, Ronald
AU - Estrada, Marta F.
AU - Vidic, Suzana
AU - Davies, Emma J.
AU - Osswald, Annika
AU - Barbier, Michael
AU - Santo, Vítor E.
AU - Gjerde, Kjersti
AU - Van Zoggel, Hanneke J.A.A.
AU - Blom, Sami
AU - Dong, Meng
AU - Närhi, Katja
AU - Boghaert, Erwin
AU - Brito, Catarina
AU - Chong, Yolanda
AU - Sommergruber, Wolfgang
AU - Van Der Kuip, Heiko
AU - Van Weerden, Wytske M.
AU - Verschuren, Emmy W.
AU - Hickman, John
AU - Graeser, Ralph
PY - 2017/11/21
Y1 - 2017/11/21
N2 - Two-dimensional (2D) culture of cancer cells in vitro does not recapitulate the three-dimensional (3D) architecture, heterogeneity and complexity of human tumors. More representative models are required that better reflect key aspects of tumor biology. These are essential studies of cancer biology and immunology as well as for target validation and drug discovery. The Innovative Medicines Initiative (IMI) consortium PREDECT (www.predect.eu) characterized in vitro models of three solid tumor types with the goal to capture elements of tumor complexity and heterogeneity. 2D culture and 3D mono-and stromal co-cultures of increasing complexity, and precision-cut tumor slice models were established. Robust protocols for the generation of these platforms are described. Tissue microarrays were prepared from all the models, permitting immunohistochemical analysis of individual cells, capturing heterogeneity. 3D cultures were also characterized using image analysis. Detailed step-by-step protocols, exemplary datasets from the 2D, 3D, and slice models, and refined analytical methods were established and are presented.
AB - Two-dimensional (2D) culture of cancer cells in vitro does not recapitulate the three-dimensional (3D) architecture, heterogeneity and complexity of human tumors. More representative models are required that better reflect key aspects of tumor biology. These are essential studies of cancer biology and immunology as well as for target validation and drug discovery. The Innovative Medicines Initiative (IMI) consortium PREDECT (www.predect.eu) characterized in vitro models of three solid tumor types with the goal to capture elements of tumor complexity and heterogeneity. 2D culture and 3D mono-and stromal co-cultures of increasing complexity, and precision-cut tumor slice models were established. Robust protocols for the generation of these platforms are described. Tissue microarrays were prepared from all the models, permitting immunohistochemical analysis of individual cells, capturing heterogeneity. 3D cultures were also characterized using image analysis. Detailed step-by-step protocols, exemplary datasets from the 2D, 3D, and slice models, and refined analytical methods were established and are presented.
UR - http://www.scopus.com/inward/record.url?scp=85034853724&partnerID=8YFLogxK
U2 - 10.1038/sdata.2017.170
DO - 10.1038/sdata.2017.170
M3 - Article
C2 - 29160867
AN - SCOPUS:85034853724
VL - 4
JO - Scientific Data
JF - Scientific Data
SN - 2052-4463
M1 - 170170
ER -
