TY - JOUR
T1 - Cytotoxicity profiling of choline chloride-based natural deep eutectic solvents
AU - Popović, Boris M.
AU - Gligorijević, Nevenka
AU - Aranđelović, Sandra
AU - Macedo, Ana Catarina
AU - Jurić, Tatjana
AU - Uka, Denis
AU - Mocko-Blažek, Karolina
AU - Serra, Ana Teresa
N1 - Funding Information:
This research was financially supported by the Science Fund of the Republic of Serbia, #grant no. 7731993, Active Pharmaceutical Ingredient Deep Eutectic Solvents as Novel Therapeutic Agents and Food Supplements – APIDES, and the Ministry of Education, Science and Technological Development of the Republic of Serbia, grant no. 451-03-68/2022-14/200117 and grant no. 451-03-68/2022-14/200043.
Publisher Copyright:
© 2023 The Royal Society of Chemistry.
PY - 2023/1/25
Y1 - 2023/1/25
N2 - This study aims to examine in detail for the first time the cytotoxic profile of twelve choline chloride-based deep eutectic solvents (NADES) against HT-29, Caco-2, MCF-7, and MRC-5 cell lines. All NADES systems were synthesized by microwave synthesis using choline chloride as a hydrogen bond acceptor (HBA) and selected sugars, alcohols, organic acids, and urea as hydrogen bond donors (HBD) with the addition of 20% water (w/w) to all systems. It was observed that the cytotoxic effect predominantly depended on the structure of HBD. Acidic systems, where HBDs were organic acids showed the highest cytotoxic effects in all investigated cell lines. The cytotoxicity depended mostly on the concentration of the NADES system in the cell medium as well as on the chemical constitution of the investigated systems. The highest cytotoxic effects showed acidic systems, especially to the HT-29 cell line. The EC50 value for the citric acid-based system was 3.91 mg mL−1 for the HT-29 cell line which was the most vulnerable to acidic NADES systems.
AB - This study aims to examine in detail for the first time the cytotoxic profile of twelve choline chloride-based deep eutectic solvents (NADES) against HT-29, Caco-2, MCF-7, and MRC-5 cell lines. All NADES systems were synthesized by microwave synthesis using choline chloride as a hydrogen bond acceptor (HBA) and selected sugars, alcohols, organic acids, and urea as hydrogen bond donors (HBD) with the addition of 20% water (w/w) to all systems. It was observed that the cytotoxic effect predominantly depended on the structure of HBD. Acidic systems, where HBDs were organic acids showed the highest cytotoxic effects in all investigated cell lines. The cytotoxicity depended mostly on the concentration of the NADES system in the cell medium as well as on the chemical constitution of the investigated systems. The highest cytotoxic effects showed acidic systems, especially to the HT-29 cell line. The EC50 value for the citric acid-based system was 3.91 mg mL−1 for the HT-29 cell line which was the most vulnerable to acidic NADES systems.
UR - https://www.scopus.com/pages/publications/85147246277
U2 - 10.1039/d2ra07488e
DO - 10.1039/d2ra07488e
M3 - Article
AN - SCOPUS:85147246277
SN - 2046-2069
VL - 13
SP - 3520
EP - 3527
JO - RSC Advances
JF - RSC Advances
IS - 6
ER -