TY - JOUR
T1 - Cyclooxygenase inhibition with curcumin in Helicobacter pylori infection
AU - Santos, António Mário
AU - Lopes, Teresa
AU - Oleastro, Mónica
AU - Pereira, Teresa
AU - Alves, Carolina Coimbra
AU - Seixas, Elsa
AU - Chaves, Paula
AU - Machado, Jorge
AU - Guerreiro, António Sousa
N1 - Funding Information:
This work was supported by PTDC/SAU-OSM/66323/2006 research grant from Funda??o para a Ci?ncia e a Tecnologia (FCT), Portugal. The datasets generated used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Funding Information:
This work was supported by PTDC/SAU-OSM/66323/2006 research grant from Fundação para a Ciência e a Tecnologia (FCT), Portugal.
Publisher Copyright:
© 2018, The Author(s).
PY - 2018/12
Y1 - 2018/12
N2 - Background: Helicobacter pylori (H. pylori) infection increases the expression of cyclooxygenase-2 (COX-2) on the host’s gastric mucosa. The inhibition of COX-2 activity with natural products would be a major advantage. This study aims to clarify the possible effect of curcumin on COX-2 inhibition in gastric mucosa of H. pylori infected mice. Methods: We inoculated 30 pathogen-free male C57BL/6 mice with H. pylori (SS1 Sidney strain) that were randomly divided in two different groups: infected group (IG) treated with phosphate-buffered saline (PBS) (n = 15) and infected group treated with curcumin 500 mg/kg (IG + C) (n = 15). A group of 15 non-infected mice were used as control (CG). Two weeks post-infection, both IG and CG groups received 0.5 ml of PBS, while the IG + C group received curcumin for 6, 18, and 27 weeks. Results: The analysis by immunohistochemistry and by PCR array at 6, 18, and 27 weeks post-infection showed a significant increase on COX-2 expression on the IG mice compared to the CG mice. The group treated with curcumin (IG + C) showed a significant downregulation of COX-2 at all points of the experiment, when compared to the IG + C mice. Conclusion: Chronic H. pylori infection induces a significant increase in COX-2 expression. Treatment with curcumin significantly decreases the COX-2 expression, and the addition of curcumin to the diet may be an interesting approach for areas of high H. pylori prevalence.
AB - Background: Helicobacter pylori (H. pylori) infection increases the expression of cyclooxygenase-2 (COX-2) on the host’s gastric mucosa. The inhibition of COX-2 activity with natural products would be a major advantage. This study aims to clarify the possible effect of curcumin on COX-2 inhibition in gastric mucosa of H. pylori infected mice. Methods: We inoculated 30 pathogen-free male C57BL/6 mice with H. pylori (SS1 Sidney strain) that were randomly divided in two different groups: infected group (IG) treated with phosphate-buffered saline (PBS) (n = 15) and infected group treated with curcumin 500 mg/kg (IG + C) (n = 15). A group of 15 non-infected mice were used as control (CG). Two weeks post-infection, both IG and CG groups received 0.5 ml of PBS, while the IG + C group received curcumin for 6, 18, and 27 weeks. Results: The analysis by immunohistochemistry and by PCR array at 6, 18, and 27 weeks post-infection showed a significant increase on COX-2 expression on the IG mice compared to the CG mice. The group treated with curcumin (IG + C) showed a significant downregulation of COX-2 at all points of the experiment, when compared to the IG + C mice. Conclusion: Chronic H. pylori infection induces a significant increase in COX-2 expression. Treatment with curcumin significantly decreases the COX-2 expression, and the addition of curcumin to the diet may be an interesting approach for areas of high H. pylori prevalence.
KW - COX-2
KW - Curcumin
KW - Experimental model
KW - H. pylori
UR - http://www.scopus.com/inward/record.url?scp=85065155872&partnerID=8YFLogxK
U2 - 10.1186/s41110-018-0070-5
DO - 10.1186/s41110-018-0070-5
M3 - Article
AN - SCOPUS:85065155872
VL - 43
JO - Nutrire
JF - Nutrire
SN - 1519-8928
IS - 1
M1 - 7
ER -
