TY - JOUR
T1 - Cutinase activity and enantioselectivity in supercritical fluids
AU - Fontes, Nuno
AU - Almeida, M. Conceição
AU - Garcia, Sílvia
AU - Peres, Célia
AU - Grave, João
AU - Aires-Barros, M. Raquel
AU - Soares, Cláudio M.
AU - Cabral, Joaquim M.S.
AU - Maycock, Christopher D.
AU - Barreiros, Susana
PY - 1998/12/1
Y1 - 1998/12/1
N2 - We studied the performance of Fusarium solani pisi cutinase, immobilized on a zeolite, in supercritical carbon dioxide and ethylene. Water activity had a strong effect on the catalytic activity of the enzyme, unlike pressure to 300 bar. The enzyme was very selective towards one of the isomers of 1-phenylethanol, with an enantiomeric excess of virtually 100 % which did not depend on water activity, pressure or solvent. Computer modeling of the structures of transition states for the two enantiomers led to structural differences which explained the preference of the enzyme for the (R)-isomer.
AB - We studied the performance of Fusarium solani pisi cutinase, immobilized on a zeolite, in supercritical carbon dioxide and ethylene. Water activity had a strong effect on the catalytic activity of the enzyme, unlike pressure to 300 bar. The enzyme was very selective towards one of the isomers of 1-phenylethanol, with an enantiomeric excess of virtually 100 % which did not depend on water activity, pressure or solvent. Computer modeling of the structures of transition states for the two enantiomers led to structural differences which explained the preference of the enzyme for the (R)-isomer.
UR - http://www.scopus.com/inward/record.url?scp=77957057279&partnerID=8YFLogxK
U2 - 10.1016/S0921-0423(98)80072-5
DO - 10.1016/S0921-0423(98)80072-5
M3 - Article
AN - SCOPUS:77957057279
VL - 15
SP - 483
EP - 486
JO - Progress in Biotechnology
JF - Progress in Biotechnology
SN - 0921-0423
IS - C
ER -