Current methods to analyse lysosome morphology, positioning, motility and function

Duarte C Barral; Leopoldo Staiano; Cláudia Guimas Almeida; Dan F Cutler; Emily R Eden; Clare E Futter; Antony Galione; André R A Marques; Diego Luis Medina; Gennaro Napolitano; Carmine Settembre; Otília V Vieira; Johannes M F G Aerts; Peace Atakpa-Adaji; Gemma Bruno; Antonella Capuozzo; Elvira De Leonibus; Chiara Di Malta; Cristina Escrevente; Alessandra Esposito; Paolo Grumati; Michael J Hall; Rita O Teodoro; Susana S Lopes; J Paul Luzio; Jlenia Monfregola; Sandro Montefusco; Frances M Platt; Roman Polishuck; Maria De Risi; Irene Sambri; Chiara Soldati; Miguel C Seabra

doi:10.1111/tra.12839

Current methods to analyse lysosome morphology, positioning, motility and function

Duarte C Barral, Leopoldo Staiano, Cláudia Guimas Almeida, Dan F Cutler, Emily R Eden, Clare E Futter, Antony Galione, André R A Marques, Diego Luis Medina, Gennaro Napolitano, Carmine Settembre, Otília V Vieira, Johannes M F G Aerts, Peace Atakpa-Adaji, Gemma Bruno, Antonella Capuozzo, Elvira De Leonibus, Chiara Di Malta, Cristina Escrevente, Alessandra EspositoPaolo Grumati, Michael J Hall, Rita O Teodoro, Susana S Lopes, J Paul Luzio, Jlenia Monfregola, Sandro Montefusco, Frances M Platt, Roman Polishuck, Maria De Risi, Irene Sambri, Chiara Soldati, Miguel C Seabra

Research output: Contribution to journal › Review article › peer-review

61 Citations (Scopus)

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Abstract

Since the discovery of lysosomes more than 70 years ago, much has been learned about the functions of these organelles. Lysosomes were regarded as exclusively degradative organelles, but more recent research has revealed that they play essential roles in several other cellular functions, such as nutrient sensing, intracellular signalling, and metabolism. Methodological advances played a key part in generating our current knowledge about the biology of this multifaceted organelle. In this review, we cover current methods used to analyse lysosome morphology, positioning, motility, and function. We highlight the principles behind these methods, the methodological strategies, and their advantages and limitations. To extract accurate information and avoid misinterpretations, we discuss the best strategies to identify lysosomes and assess their characteristics and functions. With this review, we aim to stimulate an increase in the quantity and quality of research on lysosomes and further ground-breaking discoveries on an organelle that continues to surprise and excite cell biologists.

Original language	English
Pages (from-to)	238-269
Journal	Traffic
Volume	23
Issue number	5
Early online date	28 Mar 2022
DOIs	https://doi.org/10.1111/tra.12839
Publication status	Published - May 2022

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Traffic - 2022 - Barral - Current methods to analyze lysosome morphology positioning motility and functionFinal published version, 41.9 MBLicence: CC BY

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Barral, D. C., Staiano, L., Almeida, C. G., Cutler, D. F., Eden, E. R., Futter, C. E., Galione, A., Marques, A. R. A., Medina, D. L., Napolitano, G., Settembre, C., Vieira, O. V., Aerts, J. M. F. G., Atakpa-Adaji, P., Bruno, G., Capuozzo, A., De Leonibus, E., Di Malta, C., Escrevente, C., ... Seabra, M. C. (2022). Current methods to analyse lysosome morphology, positioning, motility and function. Traffic, 23(5), 238-269. Advance online publication. https://doi.org/10.1111/tra.12839

@article{fd11eecb4fe24aea9bfef6f5308ad459,

title = "Current methods to analyse lysosome morphology, positioning, motility and function",

abstract = "Since the discovery of lysosomes more than 70 years ago, much has been learned about the functions of these organelles. Lysosomes were regarded as exclusively degradative organelles, but more recent research has revealed that they play essential roles in several other cellular functions, such as nutrient sensing, intracellular signalling, and metabolism. Methodological advances played a key part in generating our current knowledge about the biology of this multifaceted organelle. In this review, we cover current methods used to analyse lysosome morphology, positioning, motility, and function. We highlight the principles behind these methods, the methodological strategies, and their advantages and limitations. To extract accurate information and avoid misinterpretations, we discuss the best strategies to identify lysosomes and assess their characteristics and functions. With this review, we aim to stimulate an increase in the quantity and quality of research on lysosomes and further ground-breaking discoveries on an organelle that continues to surprise and excite cell biologists.",

author = "Barral, {Duarte C} and Leopoldo Staiano and Almeida, {Cl{\'a}udia Guimas} and Cutler, {Dan F} and Eden, {Emily R} and Futter, {Clare E} and Antony Galione and Marques, {Andr{\'e} R A} and Medina, {Diego Luis} and Gennaro Napolitano and Carmine Settembre and Vieira, {Ot{\'i}lia V} and Aerts, {Johannes M F G} and Peace Atakpa-Adaji and Gemma Bruno and Antonella Capuozzo and {De Leonibus}, Elvira and {Di Malta}, Chiara and Cristina Escrevente and Alessandra Esposito and Paolo Grumati and Hall, {Michael J} and Teodoro, {Rita O} and Lopes, {Susana S} and Luzio, {J Paul} and Jlenia Monfregola and Sandro Montefusco and Platt, {Frances M} and Roman Polishuck and {De Risi}, Maria and Irene Sambri and Chiara Soldati and Seabra, {Miguel C}",

note = "Funding: We thank Liliana Bento for valuable assistance in formatting the manuscript. This article was supported by the LYSOCIL project. This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 811087. CGA has funding from Maratona da Sa{\'u}de 2016 and Funda{\c c}ao para a Ci{\^e}ncia e a Tecnologia ˜ , I.P. (CEECIND/00410/2017). FMP is a Wellcome Investigator in Science and a Royal Society Wilson merit award holder.",

year = "2022",

month = may,

doi = "10.1111/tra.12839",

language = "English",

volume = "23",

pages = "238--269",

journal = "Traffic",

issn = "1398-9219",

publisher = "Blackwell Publishing Ltd",

number = "5",

}

Barral, DC, Staiano, L, Almeida, CG, Cutler, DF, Eden, ER, Futter, CE, Galione, A, Marques, ARA, Medina, DL, Napolitano, G, Settembre, C, Vieira, OV, Aerts, JMFG, Atakpa-Adaji, P, Bruno, G, Capuozzo, A, De Leonibus, E, Di Malta, C, Escrevente, C, Esposito, A, Grumati, P, Hall, MJ , Teodoro, RO , Lopes, SS, Luzio, JP, Monfregola, J, Montefusco, S, Platt, FM, Polishuck, R, De Risi, M, Sambri, I, Soldati, C & Seabra, MC 2022, 'Current methods to analyse lysosome morphology, positioning, motility and function', Traffic, vol. 23, no. 5, pp. 238-269. https://doi.org/10.1111/tra.12839

TY - JOUR

T1 - Current methods to analyse lysosome morphology, positioning, motility and function

AU - Barral, Duarte C

AU - Staiano, Leopoldo

AU - Almeida, Cláudia Guimas

AU - Cutler, Dan F

AU - Eden, Emily R

AU - Futter, Clare E

AU - Galione, Antony

AU - Marques, André R A

AU - Medina, Diego Luis

AU - Napolitano, Gennaro

AU - Settembre, Carmine

AU - Vieira, Otília V

AU - Aerts, Johannes M F G

AU - Atakpa-Adaji, Peace

AU - Bruno, Gemma

AU - Capuozzo, Antonella

AU - De Leonibus, Elvira

AU - Di Malta, Chiara

AU - Escrevente, Cristina

AU - Esposito, Alessandra

AU - Grumati, Paolo

AU - Hall, Michael J

AU - Teodoro, Rita O

AU - Lopes, Susana S

AU - Luzio, J Paul

AU - Monfregola, Jlenia

AU - Montefusco, Sandro

AU - Platt, Frances M

AU - Polishuck, Roman

AU - De Risi, Maria

AU - Sambri, Irene

AU - Soldati, Chiara

AU - Seabra, Miguel C

N1 - Funding: We thank Liliana Bento for valuable assistance in formatting the manuscript. This article was supported by the LYSOCIL project. This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 811087. CGA has funding from Maratona da Saúde 2016 and Fundaçao para a Ciência e a Tecnologia ˜ , I.P. (CEECIND/00410/2017). FMP is a Wellcome Investigator in Science and a Royal Society Wilson merit award holder.

PY - 2022/5

Y1 - 2022/5

N2 - Since the discovery of lysosomes more than 70 years ago, much has been learned about the functions of these organelles. Lysosomes were regarded as exclusively degradative organelles, but more recent research has revealed that they play essential roles in several other cellular functions, such as nutrient sensing, intracellular signalling, and metabolism. Methodological advances played a key part in generating our current knowledge about the biology of this multifaceted organelle. In this review, we cover current methods used to analyse lysosome morphology, positioning, motility, and function. We highlight the principles behind these methods, the methodological strategies, and their advantages and limitations. To extract accurate information and avoid misinterpretations, we discuss the best strategies to identify lysosomes and assess their characteristics and functions. With this review, we aim to stimulate an increase in the quantity and quality of research on lysosomes and further ground-breaking discoveries on an organelle that continues to surprise and excite cell biologists.

AB - Since the discovery of lysosomes more than 70 years ago, much has been learned about the functions of these organelles. Lysosomes were regarded as exclusively degradative organelles, but more recent research has revealed that they play essential roles in several other cellular functions, such as nutrient sensing, intracellular signalling, and metabolism. Methodological advances played a key part in generating our current knowledge about the biology of this multifaceted organelle. In this review, we cover current methods used to analyse lysosome morphology, positioning, motility, and function. We highlight the principles behind these methods, the methodological strategies, and their advantages and limitations. To extract accurate information and avoid misinterpretations, we discuss the best strategies to identify lysosomes and assess their characteristics and functions. With this review, we aim to stimulate an increase in the quantity and quality of research on lysosomes and further ground-breaking discoveries on an organelle that continues to surprise and excite cell biologists.

U2 - 10.1111/tra.12839

DO - 10.1111/tra.12839

M3 - Review article

C2 - 35343629

SN - 1398-9219

VL - 23

SP - 238

EP - 269

JO - Traffic

JF - Traffic

IS - 5

ER -

Current methods to analyse lysosome morphology, positioning, motility and function

Abstract

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