Culture media and sampling collection method for aspergillus spp. Assessment: tackling the gap between recommendations and the scientific evidence

Carla Viegas, Marta Dias, Elisabete Carolino, Raquel Sabino

Research output: Contribution to journalArticle

2 Downloads (Pure)

Abstract

Culturing is still the most widely used method for determining fungal growth. Thus, is important to identify the most suitable culture media to assess Aspergillus spp. The aim of this study was to analyze data obtained from previous studies, aiming at identifying the most suitable culture media (malt extract agar (MEA) or dichloran-glycerol agar (DG18) to assess Aspergillus spp. isolation and growth. This study was conducted by using environmental samples (n = 1153). Most of the active sampling methods (air samples) were impacted directly onto both culture media. As for passive sampling methods, fungi were extracted from environmental matrices inoculated onto both media. Overall, total Aspergillus counts were higher in MEA (n = 617, 53.5%) than in DG18 (n = 536, 46.5%). Regarding Aspergillus sections, significant associations were detected with the media (χ2 (7) = 241.118, p < 0.001), the sampling approach (p < 0.001, 95% CI = (0.3 × 10−4 ), and the indoor environment (p < 0.001, 95% CI = (0.3 × 10−4 )). As such, sampling approach and the culture media should be accurately selected when dealing with Aspergillus spp. exposure assessment.

Original languageEnglish
Article number23
Pages (from-to)1-12
Number of pages12
JournalAtmosphere
Volume12
Issue number1
DOIs
Publication statusPublished - Jan 2021

Keywords

  • Aspergillus spp
  • DG18
  • Exposure
  • Indoor
  • MEA
  • Sampling

Fingerprint Dive into the research topics of 'Culture media and sampling collection method for aspergillus spp. Assessment: tackling the gap between recommendations and the scientific evidence'. Together they form a unique fingerprint.

Cite this