TY - JOUR
T1 - Culture media and sampling collection method for aspergillus spp. Assessment
T2 - tackling the gap between recommendations and the scientific evidence
AU - Viegas, Carla
AU - Dias, Marta
AU - Carolino, Elisabete
AU - Sabino, Raquel
PY - 2021/1
Y1 - 2021/1
N2 - Culturing is still the most widely used method for determining fungal growth. Thus, is important to identify the most suitable culture media to assess Aspergillus spp. The aim of this study was to analyze data obtained from previous studies, aiming at identifying the most suitable culture media (malt extract agar (MEA) or dichloran-glycerol agar (DG18) to assess Aspergillus spp. isolation and growth. This study was conducted by using environmental samples (n = 1153). Most of the active sampling methods (air samples) were impacted directly onto both culture media. As for passive sampling methods, fungi were extracted from environmental matrices inoculated onto both media. Overall, total Aspergillus counts were higher in MEA (n = 617, 53.5%) than in DG18 (n = 536, 46.5%). Regarding Aspergillus sections, significant associations were detected with the media (χ2 (7) = 241.118, p < 0.001), the sampling approach (p < 0.001, 95% CI = (0.3 × 10−4 ), and the indoor environment (p < 0.001, 95% CI = (0.3 × 10−4 )). As such, sampling approach and the culture media should be accurately selected when dealing with Aspergillus spp. exposure assessment.
AB - Culturing is still the most widely used method for determining fungal growth. Thus, is important to identify the most suitable culture media to assess Aspergillus spp. The aim of this study was to analyze data obtained from previous studies, aiming at identifying the most suitable culture media (malt extract agar (MEA) or dichloran-glycerol agar (DG18) to assess Aspergillus spp. isolation and growth. This study was conducted by using environmental samples (n = 1153). Most of the active sampling methods (air samples) were impacted directly onto both culture media. As for passive sampling methods, fungi were extracted from environmental matrices inoculated onto both media. Overall, total Aspergillus counts were higher in MEA (n = 617, 53.5%) than in DG18 (n = 536, 46.5%). Regarding Aspergillus sections, significant associations were detected with the media (χ2 (7) = 241.118, p < 0.001), the sampling approach (p < 0.001, 95% CI = (0.3 × 10−4 ), and the indoor environment (p < 0.001, 95% CI = (0.3 × 10−4 )). As such, sampling approach and the culture media should be accurately selected when dealing with Aspergillus spp. exposure assessment.
KW - Aspergillus spp
KW - DG18
KW - Exposure
KW - Indoor
KW - MEA
KW - Sampling
UR - http://www.scopus.com/inward/record.url?scp=85098635480&partnerID=8YFLogxK
U2 - 10.3390/atmos12010023
DO - 10.3390/atmos12010023
M3 - Article
AN - SCOPUS:85098635480
VL - 12
SP - 1
EP - 12
JO - Atmosphere
JF - Atmosphere
SN - 2073-4433
IS - 1
M1 - 23
ER -