TY - JOUR
T1 - Cu, Zn- Superoxide dismutase liposomal dry powder formulations production using supercritical CO2-assisted spray-drying
T2 - A proof-of-concept
AU - Costa, Clarinda
AU - Casimiro, Teresa
AU - Corvo, M. Luísa
AU - Aguiar-Ricardo, Ana
N1 - info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT#
info:eu-repo/grantAgreement/FCT/OE/PD%2FBD%2F142880%2F2018/PT#
info:eu-repo/grantAgreement/FCT/OE/COVID%2FBD%2F152744%2F2022/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04138%2F2020/PT#
Funding Information:
C. Costa thanks FCT (Fundação para a Ciência e Tecnologia) and ESF (European Social Fund) through POCH (Programa Operacional Capital Humano) for Project PD/00184/2012-PDQS . C. Costa and A. Aguiar-Ricardo acknowledge also CA18224 GREENERING (“Green Chemical Engineering Network towards upscaling sustainable processes”). COST Actions are funded within the EU Horizon 2020 Programme . M.L. Corvo is grateful for the financial support of the Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa , Lisbon, Portugal, which is supported in part by UID/DTP/04138/2020 from FCT/MCTES , Portugal. The authors are grateful to Prof. M. Dionísio for the DSC facility.
Publisher Copyright:
© 2023
PY - 2023/9
Y1 - 2023/9
N2 - Enzyme-based inhalable therapeutics for lung inflammation are gaining interest as an alternative to long-term corticosteroids treatments. However, enzymes have poor pharmacokinetics. Encapsulating enzymes in liposomes can increase their half-live and modify their biodistribution. But both liposomes and enzymes are susceptible to destabilization during storage. This drawback can be surpassed, by converting liposomal suspension into solid dosage forms for different administration routes, including inhalation. In this study, Cu, Zn- superoxide dismutase (SOD) was encapsulated in liposomes, then dried using supercritical CO2-assisted spray-drying to make SOD-loaded liposomal dry powder formulations (SOD_Lip-DPFs). Upon resuspension in water, liposomes maintained structural integrity, with 99% SOD encapsulation efficiency and preserved enzymatic activity. Stability studies showed that SOD_Lip-DPFs maintained liposomal and enzyme stability for 50 days at 40% relative humidity. This offers a stable and efficient delivery system for enzyme-based inhalable therapeutics.
AB - Enzyme-based inhalable therapeutics for lung inflammation are gaining interest as an alternative to long-term corticosteroids treatments. However, enzymes have poor pharmacokinetics. Encapsulating enzymes in liposomes can increase their half-live and modify their biodistribution. But both liposomes and enzymes are susceptible to destabilization during storage. This drawback can be surpassed, by converting liposomal suspension into solid dosage forms for different administration routes, including inhalation. In this study, Cu, Zn- superoxide dismutase (SOD) was encapsulated in liposomes, then dried using supercritical CO2-assisted spray-drying to make SOD-loaded liposomal dry powder formulations (SOD_Lip-DPFs). Upon resuspension in water, liposomes maintained structural integrity, with 99% SOD encapsulation efficiency and preserved enzymatic activity. Stability studies showed that SOD_Lip-DPFs maintained liposomal and enzyme stability for 50 days at 40% relative humidity. This offers a stable and efficient delivery system for enzyme-based inhalable therapeutics.
KW - Cu, Zn – superoxide dismutase
KW - Inflammation
KW - Pulmonary delivery
KW - Solid dosage forms
KW - Therapeutic enzymes
UR - http://www.scopus.com/inward/record.url?scp=85160038235&partnerID=8YFLogxK
U2 - 10.1016/j.supflu.2023.105991
DO - 10.1016/j.supflu.2023.105991
M3 - Article
AN - SCOPUS:85160038235
SN - 0896-8446
VL - 200
JO - Journal of Supercritical Fluids
JF - Journal of Supercritical Fluids
M1 - 105991
ER -