Crystallization and preliminary X-ray diffraction analysis of two pH-dependent forms of a di-haem cytochrome c peroxidase from Pseudomonas nautica

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Two crystal forms of cytochrome c peroxidase from Pseudomonas nautica were obtained, one at pH 4.0 using sodium citrate as precipitant and another at pH 5.3 using ammonium phosphate and sodium citrate as precipitants. The two forms belong to different space groups P3121 (pH 4.0) and P6422 (pH 5.3), with unit-cell parameters a = b = 114.5, c = 90.7 Å and a = b = 151.0, c = 155.9 Å, respectively. Several complete data sets were collected using synchrotron radiation at ESRF and Cu Kα X-ray radiation from a rotating-anode generator. These results will contribute to clarifying the haem transitions occurring during peroxidatic reaction and the required electron-transfer processes and to elucidating the catalytic mechanism of the enzyme and the role of calcium in the activation process.

Original languageEnglish
Pages (from-to)697-699
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume58
Issue number4
DOIs
Publication statusPublished - 29 Apr 2002

Fingerprint Dive into the research topics of 'Crystallization and preliminary X-ray diffraction analysis of two pH-dependent forms of a di-haem cytochrome c peroxidase from Pseudomonas nautica'. Together they form a unique fingerprint.

Cite this