Crystallization and preliminary X-ray diffraction analysis of the di-haem cytochrome c peroxidase from Pseudomonas stutzeri

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Abstract

Crystals of cytochrome c peroxidase from Pseudomonas stutzeri were obtained using sodium citrate and PEG 8000 as precipitants. A complete data set was collected to a resolution of 1.6. Å under cryogenic conditions using synchrotron radiation at the ESRF. The crystals belong to space group P21, with unit-cell parameters a = 69.29, b = 143.31, c = 76.83 Å, β = 100.78°. Four CCP molecules were found in the asymmetric unit, corresponding to a pair of dimers related by local dyads. The crystal packing in the structure shows that the functional dimers can dimerize, as suggested by previous biochemical studies.

Original languageEnglish
Pages (from-to)345-347
Number of pages3
JournalActa Crystallographica - Section D Biological Crystallography
Volume59
Issue number2
DOIs
Publication statusPublished - 1 Feb 2003

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