TY - JOUR
T1 - Crystallization and preliminary X-ray diffraction analysis of the di-haem cytochrome c peroxidase from Pseudomonas stutzeri
AU - Bonifácio, Cecília
AU - Cunha, Carlos A.
AU - Miller, Axel
AU - Timóteo, Cristina G.
AU - Dias, João M.
AU - Moura, Isabel
AU - Romão, Maria João
PY - 2003/2/1
Y1 - 2003/2/1
N2 - Crystals of cytochrome c peroxidase from Pseudomonas stutzeri were obtained using sodium citrate and PEG 8000 as precipitants. A complete data set was collected to a resolution of 1.6. Å under cryogenic conditions using synchrotron radiation at the ESRF. The crystals belong to space group P21, with unit-cell parameters a = 69.29, b = 143.31, c = 76.83 Å, β = 100.78°. Four CCP molecules were found in the asymmetric unit, corresponding to a pair of dimers related by local dyads. The crystal packing in the structure shows that the functional dimers can dimerize, as suggested by previous biochemical studies.
AB - Crystals of cytochrome c peroxidase from Pseudomonas stutzeri were obtained using sodium citrate and PEG 8000 as precipitants. A complete data set was collected to a resolution of 1.6. Å under cryogenic conditions using synchrotron radiation at the ESRF. The crystals belong to space group P21, with unit-cell parameters a = 69.29, b = 143.31, c = 76.83 Å, β = 100.78°. Four CCP molecules were found in the asymmetric unit, corresponding to a pair of dimers related by local dyads. The crystal packing in the structure shows that the functional dimers can dimerize, as suggested by previous biochemical studies.
UR - http://www.scopus.com/inward/record.url?scp=0037319850&partnerID=8YFLogxK
U2 - 10.1107/S0907444902020760
DO - 10.1107/S0907444902020760
M3 - Article
C2 - 12554948
AN - SCOPUS:0037319850
SN - 0907-4449
VL - 59
SP - 345
EP - 347
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 2
ER -