Crystallization and preliminary X-ray analysis of a membrane-bound nitrite reductase from Desulfovibrio desulfuricans ATCC 27774

João Miguel L. Dias; Cunha Carlos; Susana Marujo Teixeira; Maria Gabriela Machado de Almeida; Cristina Costa; Jorge Manuel Pinto Lampreia Pereira; José J. G. Moura; Isabel Maria Andrade Martins Galhardas de Moura; Maria João Romão

doi:10.1107/S0907444999016066

Crystallization and preliminary X-ray analysis of a membrane-bound nitrite reductase from Desulfovibrio desulfuricans ATCC 27774

João Miguel L. Dias, Cunha Carlos, Susana Marujo Teixeira, Maria Gabriela Machado de Almeida, Cristina Costa, Jorge Manuel Pinto Lampreia Pereira, José J. G. Moura, Isabel Maria Andrade Martins Galhardas de Moura, Maria João Romão

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Nitrite reductase from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 is a multihaem (type c) membrane-bound enzyme that catalyzes the dissimilatory conversion of nitrite to ammonia. Crystals of the oxidized form of this enzyme were obtained using PEG and CaCl2 as precipitants in the presence of 3-(decylmethylammonium)propane-1-sulfonate and belong to the space group P2(1)2(1)2(1), With unit-cell parameters a = 78.94, b = 104.59, c = 143.18 Angstrom. A complete data set to 2.30 Angstrom resolution was collected using synchrotron radiation at the ESRF. However, the crystals may diffract to beyond 1.7 Angstrom and high-resolution data will be collected in the near future.

Original language	Unknown
Pages (from-to)	215-217
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	56
Issue number	2
DOIs	https://doi.org/10.1107/S0907444999016066
Publication status	Published - Feb 2000

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10.1107/S0907444999016066

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Dias, J. M. L., Carlos, C., Teixeira, S. M., Almeida, M. G. M. D., Costa, C., Pereira, J. M. P. L., Moura, J. J. G., Moura, I. M. A. M. G. D., & Romão, M. J. (2000). Crystallization and preliminary X-ray analysis of a membrane-bound nitrite reductase from Desulfovibrio desulfuricans ATCC 27774. Acta Crystallographica Section D: Biological Crystallography, 56(2), 215-217. https://doi.org/10.1107/S0907444999016066

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abstract = "Nitrite reductase from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 is a multihaem (type c) membrane-bound enzyme that catalyzes the dissimilatory conversion of nitrite to ammonia. Crystals of the oxidized form of this enzyme were obtained using PEG and CaCl2 as precipitants in the presence of 3-(decylmethylammonium)propane-1-sulfonate and belong to the space group P2(1)2(1)2(1), With unit-cell parameters a = 78.94, b = 104.59, c = 143.18 Angstrom. A complete data set to 2.30 Angstrom resolution was collected using synchrotron radiation at the ESRF. However, the crystals may diffract to beyond 1.7 Angstrom and high-resolution data will be collected in the near future.",

keywords = "ATCC-27774, CYTOCHROME, PROTEIN, ESCHERICHIA-COLI",

author = "Dias, {Jo{\~a}o Miguel L.} and Cunha Carlos and Teixeira, {Susana Marujo} and Almeida, {Maria Gabriela Machado de} and Cristina Costa and Pereira, {Jorge Manuel Pinto Lampreia} and Moura, {Jos{\'e} J. G.} and Moura, {Isabel Maria Andrade Martins Galhardas de} and Rom{\~a}o, {Maria Jo{\~a}o}",

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Dias, JML, Carlos, C, Teixeira, SM, Almeida, MGMD, Costa, C, Pereira, JMPL , Moura, JJG , Moura, IMAMGD & Romão, MJ 2000, 'Crystallization and preliminary X-ray analysis of a membrane-bound nitrite reductase from Desulfovibrio desulfuricans ATCC 27774', Acta Crystallographica Section D: Biological Crystallography, vol. 56, no. 2, pp. 215-217. https://doi.org/10.1107/S0907444999016066

Crystallization and preliminary X-ray analysis of a membrane-bound nitrite reductase from Desulfovibrio desulfuricans ATCC 27774. / Dias, João Miguel L.; Carlos, Cunha; Teixeira, Susana Marujo et al.
In: Acta Crystallographica Section D: Biological Crystallography, Vol. 56, No. 2, 02.2000, p. 215-217.

Research output: Contribution to journal › Article › peer-review

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