Crystallization and preliminary X-ray analysis of a membrane-bound nitrite reductase from Desulfovibrio desulfuricans ATCC 27774

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Abstract

Nitrite reductase from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 is a multihaem (type c) membrane-bound enzyme that catalyzes the dissimilatory conversion of nitrite to ammonia. Crystals of the oxidized form of this enzyme were obtained using PEG and CaCl2 as precipitants in the presence of 3-(decylmethylammonium)propane-1-sulfonate and belong to the space group P2(1)2(1)2(1), With unit-cell parameters a = 78.94, b = 104.59, c = 143.18 Angstrom. A complete data set to 2.30 Angstrom resolution was collected using synchrotron radiation at the ESRF. However, the crystals may diffract to beyond 1.7 Angstrom and high-resolution data will be collected in the near future.
Original languageUnknown
Pages (from-to)215-217
JournalActa Crystallographica Section D-Biological Crystallography
Volume56
Issue number2
DOIs
Publication statusPublished - Feb 2000

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