Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas

Américo G. Duarte; Sofia Rocha Pauleta; Isabel Maria Andrade Martins Galhardas de Moura; José João Galhardas de Moura; Maria João Romão

doi:10.1107/S1744309109023392

Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas

Américo G. Duarte, Sofia Rocha Pauleta, Isabel Maria Andrade Martins Galhardas de Moura, José João Galhardas de Moura, Maria João Romão

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The orange-coloured protein (ORP) from Desulfovibrio gigas is a 12 kDa protein that contains a novel mixed-metal sulfide cluster of the type [S2MoS2-CuS2MoS2]. Diffracting crystals of the apo form of ORP have been obtained. Data have been collected for the apo form of ORP to 2.25 angstrom resolution in-house and to beyond 2.0 angstrom resolution at ESRF, Grenoble. The crystals belonged to a trigonal space group, with unit-cell parameters a = 43, b = 43, c = 106 angstrom.

Original language	Unknown
Pages (from-to)	730-732
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	65
Issue number	NA
DOIs	https://doi.org/10.1107/S1744309109023392
Publication status	Published - 1 Jan 2009

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10.1107/S1744309109023392

Cite this

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title = "Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas",

abstract = "The orange-coloured protein (ORP) from Desulfovibrio gigas is a 12 kDa protein that contains a novel mixed-metal sulfide cluster of the type [S2MoS2-CuS2MoS2]. Diffracting crystals of the apo form of ORP have been obtained. Data have been collected for the apo form of ORP to 2.25 angstrom resolution in-house and to beyond 2.0 angstrom resolution at ESRF, Grenoble. The crystals belonged to a trigonal space group, with unit-cell parameters a = 43, b = 43, c = 106 angstrom.",

keywords = "generation, cluster, nmr, mo, cu, thermotoga-maritima, determination, structure",

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year = "2009",

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doi = "10.1107/S1744309109023392",

language = "Unknown",

volume = "65",

pages = "730--732",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

issn = "1744-3091",

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number = "NA",

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Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas. / Duarte, Américo G.; Pauleta, Sofia Rocha ; Moura, Isabel Maria Andrade Martins Galhardas de et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 65, No. NA, 01.01.2009, p. 730-732.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas

AU - Duarte, Américo G.

AU - Pauleta, Sofia Rocha

AU - Moura, Isabel Maria Andrade Martins Galhardas de

AU - Moura, José João Galhardas de

AU - Romão, Maria João

PY - 2009/1/1

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AB - The orange-coloured protein (ORP) from Desulfovibrio gigas is a 12 kDa protein that contains a novel mixed-metal sulfide cluster of the type [S2MoS2-CuS2MoS2]. Diffracting crystals of the apo form of ORP have been obtained. Data have been collected for the apo form of ORP to 2.25 angstrom resolution in-house and to beyond 2.0 angstrom resolution at ESRF, Grenoble. The crystals belonged to a trigonal space group, with unit-cell parameters a = 43, b = 43, c = 106 angstrom.

KW - generation

KW - cluster

KW - nmr

KW - mo

KW - cu

KW - thermotoga-maritima

KW - determination

KW - structure

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DO - 10.1107/S1744309109023392

M3 - Article

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SP - 730

EP - 732

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

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