The development and assessment of satellite ocean color products require quality assured in situ data representative of the variety of bio-optical regimes encountered in the different seas. The measurement program namedBio-Optical mapping of Marine Properties(BiOMaP) fulfills this requirement by using identical instruments and applying cross-site consistent methods for the characterization of seawater inherent and apparent optical properties in the various European seas. This work introduces the BiOMaP radiometric data and describes their application to the validation of primary ocean color products. Within this framework, the radiometric data are discussed through the spectral shape and amplitude of normalized water-leaving radiances (LWN). Specifically, the spectral shape is expressed through the Principal Component Analysis ofLWN(λ)/LWN(555) while the amplitude is represented byLWN(555). The resulting distribution of BiOMaP data in a three dimensional feature space demonstrates a continuity of cases across the investigated marine regions confirming a wide representativity of bio-optical regimes. The application of BiOMaP data to the validation of remote sensing reflectance from the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) and the Moderate Resolution Imaging Spectroradiometer (MODIS), indicates improved performance of the SeaWiFS Data Analysis System (SeaDAS, version 6.1) atmospheric correction. In particular, the comparison of satellite and in situ matchups in the blue spectral region shows biases of a few percent with respect to the much larger reported in studies relying on earlier SeaDAS versions. Matchup analyses, restricted to the Eastern Mediterranean, Black and Baltic Seas, indicate marked regional differences likely explained by the diversity of water and aerosol types.