Abstract
| Original language | Unknown |
|---|---|
| Pages (from-to) | 90-94 |
| Journal | Journal of Biotechnology |
| Volume | 168 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jan 2013 |
Keywords
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Coupling an universal primer to SBE combined spectral codification strategy for single nucleotide polymorphism analysis. / Lima, João Carlos dos Santos Silva E Pereira de; Baptista, Pedro Miguel Ribeiro Viana.
In: Journal of Biotechnology, Vol. 168, No. 1, 01.01.2013, p. 90-94.Research output: Contribution to journal › Article
TY - JOUR
T1 - Coupling an universal primer to SBE combined spectral codification strategy for single nucleotide polymorphism analysis
AU - Lima, João Carlos dos Santos Silva E Pereira de
AU - Baptista, Pedro Miguel Ribeiro Viana
N1 - Sem PDF
PY - 2013/1/1
Y1 - 2013/1/1
N2 - We previously reported a strategy that combines Forster resonance energy transfer (FRET) based spectral codification with a single base extension (SBE) reaction for single nucleotide sequence discrimination in solution. This strategy is capable of unequivocally detect the allele variants present in solution. To extend the use of this tool to any locus of interest, it would be required the development of an universal approach capable of combining a sequence specific SBE primer to an universal sequence labeled and optimized for spectral codification.Here, we extend this concept to a general strategy by means of a labeled universal oligonucleotide primer (donor), a sequence specific primer that allows for incorporation of the complementary acceptor labeled ddNTP, which allows discrimination the allele variant in the sample via the unambiguous FRET signature of the donor/acceptor pair
AB - We previously reported a strategy that combines Forster resonance energy transfer (FRET) based spectral codification with a single base extension (SBE) reaction for single nucleotide sequence discrimination in solution. This strategy is capable of unequivocally detect the allele variants present in solution. To extend the use of this tool to any locus of interest, it would be required the development of an universal approach capable of combining a sequence specific SBE primer to an universal sequence labeled and optimized for spectral codification.Here, we extend this concept to a general strategy by means of a labeled universal oligonucleotide primer (donor), a sequence specific primer that allows for incorporation of the complementary acceptor labeled ddNTP, which allows discrimination the allele variant in the sample via the unambiguous FRET signature of the donor/acceptor pair
KW - Spectral codification
KW - Universal SBE primer
KW - SBE
KW - FRET
KW - SNP
U2 - 10.1016/j.jbiotec.2013.08.005
DO - 10.1016/j.jbiotec.2013.08.005
M3 - Article
VL - 168
SP - 90
EP - 94
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
IS - 1
ER -