6 Citations (Scopus)

Abstract

We previously reported a strategy that combines Forster resonance energy transfer (FRET) based spectral codification with a single base extension (SBE) reaction for single nucleotide sequence discrimination in solution. This strategy is capable of unequivocally detect the allele variants present in solution. To extend the use of this tool to any locus of interest, it would be required the development of an universal approach capable of combining a sequence specific SBE primer to an universal sequence labeled and optimized for spectral codification.Here, we extend this concept to a general strategy by means of a labeled universal oligonucleotide primer (donor), a sequence specific primer that allows for incorporation of the complementary acceptor labeled ddNTP, which allows discrimination the allele variant in the sample via the unambiguous FRET signature of the donor/acceptor pair
Original languageUnknown
Pages (from-to)90-94
JournalJournal of Biotechnology
Volume168
Issue number1
DOIs
Publication statusPublished - 1 Jan 2013

Cite this

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title = "Coupling an universal primer to SBE combined spectral codification strategy for single nucleotide polymorphism analysis",
abstract = "We previously reported a strategy that combines Forster resonance energy transfer (FRET) based spectral codification with a single base extension (SBE) reaction for single nucleotide sequence discrimination in solution. This strategy is capable of unequivocally detect the allele variants present in solution. To extend the use of this tool to any locus of interest, it would be required the development of an universal approach capable of combining a sequence specific SBE primer to an universal sequence labeled and optimized for spectral codification.Here, we extend this concept to a general strategy by means of a labeled universal oligonucleotide primer (donor), a sequence specific primer that allows for incorporation of the complementary acceptor labeled ddNTP, which allows discrimination the allele variant in the sample via the unambiguous FRET signature of the donor/acceptor pair",
keywords = "Spectral codification, Universal SBE primer, SBE, FRET, SNP",
author = "Lima, {Jo{\~a}o Carlos dos Santos Silva E Pereira de} and Baptista, {Pedro Miguel Ribeiro Viana}",
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year = "2013",
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TY - JOUR

T1 - Coupling an universal primer to SBE combined spectral codification strategy for single nucleotide polymorphism analysis

AU - Lima, João Carlos dos Santos Silva E Pereira de

AU - Baptista, Pedro Miguel Ribeiro Viana

N1 - Sem PDF

PY - 2013/1/1

Y1 - 2013/1/1

N2 - We previously reported a strategy that combines Forster resonance energy transfer (FRET) based spectral codification with a single base extension (SBE) reaction for single nucleotide sequence discrimination in solution. This strategy is capable of unequivocally detect the allele variants present in solution. To extend the use of this tool to any locus of interest, it would be required the development of an universal approach capable of combining a sequence specific SBE primer to an universal sequence labeled and optimized for spectral codification.Here, we extend this concept to a general strategy by means of a labeled universal oligonucleotide primer (donor), a sequence specific primer that allows for incorporation of the complementary acceptor labeled ddNTP, which allows discrimination the allele variant in the sample via the unambiguous FRET signature of the donor/acceptor pair

AB - We previously reported a strategy that combines Forster resonance energy transfer (FRET) based spectral codification with a single base extension (SBE) reaction for single nucleotide sequence discrimination in solution. This strategy is capable of unequivocally detect the allele variants present in solution. To extend the use of this tool to any locus of interest, it would be required the development of an universal approach capable of combining a sequence specific SBE primer to an universal sequence labeled and optimized for spectral codification.Here, we extend this concept to a general strategy by means of a labeled universal oligonucleotide primer (donor), a sequence specific primer that allows for incorporation of the complementary acceptor labeled ddNTP, which allows discrimination the allele variant in the sample via the unambiguous FRET signature of the donor/acceptor pair

KW - Spectral codification

KW - Universal SBE primer

KW - SBE

KW - FRET

KW - SNP

U2 - 10.1016/j.jbiotec.2013.08.005

DO - 10.1016/j.jbiotec.2013.08.005

M3 - Article

VL - 168

SP - 90

EP - 94

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

IS - 1

ER -