6 Citations (Scopus)

Abstract

We previously reported a strategy that combines Forster resonance energy transfer (FRET) based spectral codification with a single base extension (SBE) reaction for single nucleotide sequence discrimination in solution. This strategy is capable of unequivocally detect the allele variants present in solution. To extend the use of this tool to any locus of interest, it would be required the development of an universal approach capable of combining a sequence specific SBE primer to an universal sequence labeled and optimized for spectral codification.Here, we extend this concept to a general strategy by means of a labeled universal oligonucleotide primer (donor), a sequence specific primer that allows for incorporation of the complementary acceptor labeled ddNTP, which allows discrimination the allele variant in the sample via the unambiguous FRET signature of the donor/acceptor pair
Original languageUnknown
Pages (from-to)90-94
JournalJournal of Biotechnology
Volume168
Issue number1
DOIs
Publication statusPublished - 1 Jan 2013

Keywords

    Cite this

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    title = "Coupling an universal primer to SBE combined spectral codification strategy for single nucleotide polymorphism analysis",
    abstract = "We previously reported a strategy that combines Forster resonance energy transfer (FRET) based spectral codification with a single base extension (SBE) reaction for single nucleotide sequence discrimination in solution. This strategy is capable of unequivocally detect the allele variants present in solution. To extend the use of this tool to any locus of interest, it would be required the development of an universal approach capable of combining a sequence specific SBE primer to an universal sequence labeled and optimized for spectral codification.Here, we extend this concept to a general strategy by means of a labeled universal oligonucleotide primer (donor), a sequence specific primer that allows for incorporation of the complementary acceptor labeled ddNTP, which allows discrimination the allele variant in the sample via the unambiguous FRET signature of the donor/acceptor pair",
    keywords = "Spectral codification, Universal SBE primer, SBE, FRET, SNP",
    author = "Lima, {Jo{\~a}o Carlos dos Santos Silva E Pereira de} and Baptista, {Pedro Miguel Ribeiro Viana}",
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    year = "2013",
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    doi = "10.1016/j.jbiotec.2013.08.005",
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    pages = "90--94",
    journal = "Journal of Biotechnology",
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    TY - JOUR

    T1 - Coupling an universal primer to SBE combined spectral codification strategy for single nucleotide polymorphism analysis

    AU - Lima, João Carlos dos Santos Silva E Pereira de

    AU - Baptista, Pedro Miguel Ribeiro Viana

    N1 - Sem PDF

    PY - 2013/1/1

    Y1 - 2013/1/1

    N2 - We previously reported a strategy that combines Forster resonance energy transfer (FRET) based spectral codification with a single base extension (SBE) reaction for single nucleotide sequence discrimination in solution. This strategy is capable of unequivocally detect the allele variants present in solution. To extend the use of this tool to any locus of interest, it would be required the development of an universal approach capable of combining a sequence specific SBE primer to an universal sequence labeled and optimized for spectral codification.Here, we extend this concept to a general strategy by means of a labeled universal oligonucleotide primer (donor), a sequence specific primer that allows for incorporation of the complementary acceptor labeled ddNTP, which allows discrimination the allele variant in the sample via the unambiguous FRET signature of the donor/acceptor pair

    AB - We previously reported a strategy that combines Forster resonance energy transfer (FRET) based spectral codification with a single base extension (SBE) reaction for single nucleotide sequence discrimination in solution. This strategy is capable of unequivocally detect the allele variants present in solution. To extend the use of this tool to any locus of interest, it would be required the development of an universal approach capable of combining a sequence specific SBE primer to an universal sequence labeled and optimized for spectral codification.Here, we extend this concept to a general strategy by means of a labeled universal oligonucleotide primer (donor), a sequence specific primer that allows for incorporation of the complementary acceptor labeled ddNTP, which allows discrimination the allele variant in the sample via the unambiguous FRET signature of the donor/acceptor pair

    KW - Spectral codification

    KW - Universal SBE primer

    KW - SBE

    KW - FRET

    KW - SNP

    U2 - 10.1016/j.jbiotec.2013.08.005

    DO - 10.1016/j.jbiotec.2013.08.005

    M3 - Article

    VL - 168

    SP - 90

    EP - 94

    JO - Journal of Biotechnology

    JF - Journal of Biotechnology

    SN - 0168-1656

    IS - 1

    ER -