Copper tolerance in Marinobacter hydrocarbonoclasticus - Proteomic analysis of the periplasm

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Abstract

Marinobacter hydrocarbonoclasticus was shown to have a high tolerance to copper sulphate. A minimum inhibitory concentration of 1.6 mM was determined, thus classifying Marinobacter hydrocarbonoclasticus as copper resistant. A proteomic approach based on two-dimensional gel electrophoresis provided a preliminary analysis over the differential protein expression induced by 1 mM of copper in Marinobacter hydrocarbonoclasticus. The copper stress led to a global change in the proteomic profile of the periplasmic fraction of this bacterium, involving modulation of the expression of 53 out of the close to 500 proteins detected, when compared with control growths with trace copper concentration.

Original languageEnglish
Title of host publicationMICROBES IN APPLIED RESEARCH: CURRENT ADVANCES AND CHALLENGES
EditorsA. MendezVilas
PublisherWorld Scientific Publishing Co. Pte Ltd
Pages608-612
Number of pages5
ISBN (Print)978-981-4405-03-4
DOIs
Publication statusPublished - 2012
Event4th International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld) - Malaga, Spain
Duration: 14 Sep 201116 Sep 2011

Conference

Conference4th International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld)
CountrySpain
CityMalaga
Period14/09/1116/09/11

Keywords

  • Copper tolerance
  • Marinobacter hydrocarbonoclasticus
  • proteomics
  • PSEUDOMONAS-SYRINGAE
  • ESCHERICHIA-COLI
  • HOMEOSTASIS
  • RESISTANCE
  • MECHANISMS
  • BACTERIA
  • HOMOLOGS
  • GROWTH

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