Coordinated waves of actomyosin flow and apical cell constriction immediately after wounding

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Abstract

Epithelial wound healing relies on tissue movements and cell shape changes. Our work shows that, immediately after wounding, there was a dramatic cytoskeleton remodeling consisting of a pulse of actomyosin filaments that assembled in cells around the wound edge and flowed from cell to cell toward the margin of the wound. We show that this actomyosin flow was regulated by Diaphanous and ROCK and that it elicited a wave of apical cell constriction that culminated in the formation of the leading edge actomyosin cable, a structure that is essential for wound closure. Calcium signaling played an important role in this process, as its intracellular concentration increased dramatically immediately after wounding, and down-regulation of transient receptor potential channel M, a stress-activated calcium channel, also impaired solin, protein, also impaired the wound response, indicating that cleaving the existing actin filament network is an important part of the cytoskeleton remodeling process.
Original languageEnglish
Pages (from-to)365-379
JournalJournal Of Cell Biology
Volume202
Issue number2
DOIs
Publication statusPublished - 1 Jan 2013

Keywords

  • DROSOPHILA EMBRYOS
  • MYOSIN-II
  • TISSUE REGENERATION
  • ACTIN CYTOSKELETON
  • ADHERENS JUNCTIONS
  • GENE-EXPRESSION
  • MORPHOGENESIS
  • POLARITY
  • CLOSURE
  • PATHWAY

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