Conversion of cheese whey into a fucose- and glucuronic acid-rich extracellular polysaccharide by Enterobacter A47

Sílvia Antunes, Filomena Freitas, Vítor D. Alves, Christian Grandfils, Maria A M Reis

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cheese whey was used as the sole substrate for the production of extracellular polysaccharides (EPS) by Enterobacter A47. An EPS concentration of 6.40gL-1 was reached within 3.2 days of cultivation, corresponding to a volumetric productivity of 2.00gL-1d-1. The produced EPS was mainly composed of glucuronic acid (29mol%) and fucose (29mol%), with lower contents of glucose and galactose (21mol% each) and a total acyl groups content of 32wt.%. The polymer had an average molecular weight of 1.8×106Da, with a polydispersity index of 1.2, and an intrinsic viscosity of 8.0dLg-1. EPS aqueous solutions (1.0wt.% in 0.01M NaCl, at pH 8.0) presented a shear thinning behavior with a viscosity of the first Newtonian plateau approaching 0.1Pas. This novel glucuronic acid-rich polymer possesses interesting rheological properties, which, together with its high content of glucuronic acid and fucose, two bioactive sugar monomers, confers it a great potential for use in high-value applications, such as cosmetics and pharmaceuticals.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Biotechnology
Volume210
DOIs
Publication statusPublished - 1 Sep 2015

Keywords

  • Cheese whey
  • Enterobacter A47
  • Extracellular polysaccharide (EPS)
  • Fucose
  • Glucuronic acid

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