Continuous separation of cells by balanced dielectrophoretic forces at multiple frequencies

Thomas Braschler, Nicolas Demierre, Elisabete Nascimento, Tiago Silva, Abel G. Oliva, Philippe Renaud

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

We present a particle-sorting device based on the opposition of dielectrophoretic forces. The forces are generated by an array of electrode chambers located in both sidewalls of a main flow channel. Particles with different dielectric response perceive different force magnitudes and are therefore continuously focused to different streamlines in the flow channel. We relate the particles' dielectric response to their output position in the downstream channel. We demonstrate the performance of the device by separating a mixed yeast cell population into pure fractions of viable and nonviable cells. Finally, we use the device to enrich red blood cells infected with Babesia bovis, a major pathogen in cattle and simultaneously confirm the hypothesis that infection with B. bovis causes significant changes in the dielectric response of red blood cells.

Original languageEnglish
Pages (from-to)280-286
Number of pages7
JournalLab On A Chip
Volume8
Issue number2
DOIs
Publication statusPublished - 1 Jan 2008

Keywords

  • protozoal infection
  • yeast cell
  • animal cell
  • article
  • Babesia bovis
  • cattle
  • cell population
  • cell separation
  • cell viability
  • device
  • electric activity
  • electricity
  • electrode
  • electrophoresis
  • erythrocyte
  • hypothesis
  • nonhuman
  • priority journal

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