Continuous chromatography purification of virus-based biopharmaceuticals: A shortcut design method

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Citations (Scopus)

Abstract

Novel biopharmaceutical products, such as vaccines and viral vectors, play a significant role in the development of innovative therapeutic, prophylactic, and clinical applications. However, several challenges are posed when manufacturing these products. The diversity of cell lines and the different physical and chemical properties of these biologicals require the use of different production and processing technologies. Alternative purification strategies that can improve the purification yield, such as continuous chromatography, are regarded nowadays as enabling technologies to overcome some of the bottlenecks in biomanufacturing. This chapter offers a shortcut approach to implement a semi-continuous chromatography purification of hepatitis C virus-like particles produced in insect cells with recombinant baculovirus. Although the purification is based on ion exchange chromatography, the present methodology can be extended to other types of chromatography.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages367-384
Number of pages18
DOIs
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2095
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Continuous chromatography
  • Downstream processing
  • Purification
  • Vaccines
  • Virus-like particles

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