Construction of disposable biosensors for nitrite monitoring in food and beverages

Tiago Monteiro, Célia M. Silveira, Elena Jubete, M. Gabriela Almeida

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Nitrite salts are regularly used as fertilizers and food preservatives. Excessive levels have raised awareness from public regulation agencies, since nitrite can have an adverse impact on public health and on ecological systems. Therefore, it is important to develop sensitive assays to monitor this ion in foodstuff products and waters. This work presents a nitrite biosensor based on a highly selective nitrite reducing enzyme incorporated on carbon ink based screen-printed electrodes. The resulting device enabled the quantification of nitrite in different samples (milk, water) in a straightforward way and with small error (1-6%). The sensitivity of the biosensor towards nitrite reduction under optimized conditions was 0.55 A M-1 cm-2 with a linear response in the range 0.7-370 jM.Grant: This work was supported byCiiEM - Centro de Investigaçao Interdisciplinar Egas Moniz, and Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO, which is financed by national funds from FCT/MEC (UID/Multi/ 04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). CM. Silveira and T. Monteiro acknowledge Fundaçao para a Ciencia e Tecnologia the fellowships SFRH/BPD/79566/2011 and PD/BD/109687/2015, respectively.

Original languageEnglish
Title of host publication2nd IMEKOFOODS Conference
Subtitle of host publicationPromoting Objective and Measurable Food Quality and Safety
PublisherUniversity of Sannio
Pages219-223
Number of pages5
ISBN (Electronic)9781510844957
Publication statusPublished - 1 Jan 2016
Event2nd IMEKOFOODS Conference: Promoting Objective and Measurable Food Quality and Safety 2016 - Benevento, Italy
Duration: 2 Oct 20165 Oct 2016

Conference

Conference2nd IMEKOFOODS Conference: Promoting Objective and Measurable Food Quality and Safety 2016
CountryItaly
CityBenevento
Period2/10/165/10/16

Keywords

  • Biosensor
  • Drinks
  • Nitrites
  • Screen-printed electrodes

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