Construction and characterization of an absolute deletion mutant of Escherichia coli ribonuclease II

João Piedade; Rita Zilhão; Cecília Maria Arraiano

doi:10.1016/0378-1097(95)00060-I

Construction and characterization of an absolute deletion mutant of Escherichia coli ribonuclease II

João Piedade, Rita Zilhão, Cecília Maria Arraiano

Instituto de Tecnologia Química e Biológica António Xavier (ITQB)

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

The degradation of mRNA plays a central role in the control of protein synthesis. In Escherichia coli, the rnb gene encodes ribonuclease II (RNase II), one of the two main exonucleases involved in mRNA decay. We have constructed strain CMA201, in which the rnb promoter region and the gene were deleted from the chromosome and replaced by a tet^tr cassette. This is the first rnb absolute deletion mutant that shows the complete absence of rnb-specific mRNA. This strain has growth characteristics similar to the wild-type, even though it has no RNase II activity, and it should be useful in studies of mRNA metabolism.

Original language	English
Pages (from-to)	187-193
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	127
Issue number	3
DOIs	https://doi.org/10.1016/0378-1097(95)00060-I
Publication status	Published - 1 Apr 1995

Keywords

Deletion
Escherichia coli
mRNA degradation
Ribonuclease II
rnb

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/0378-1097(95)00060-I

Cite this

@article{4d19b63e87cf425a8080ef8a4ba2d446,

title = "Construction and characterization of an absolute deletion mutant of Escherichia coli ribonuclease II",

abstract = "The degradation of mRNA plays a central role in the control of protein synthesis. In Escherichia coli, the rnb gene encodes ribonuclease II (RNase II), one of the two main exonucleases involved in mRNA decay. We have constructed strain CMA201, in which the rnb promoter region and the gene were deleted from the chromosome and replaced by a tettr cassette. This is the first rnb absolute deletion mutant that shows the complete absence of rnb-specific mRNA. This strain has growth characteristics similar to the wild-type, even though it has no RNase II activity, and it should be useful in studies of mRNA metabolism.",

keywords = "Deletion, Escherichia coli, mRNA degradation, Ribonuclease II, rnb",

author = "Jo{\~a}o Piedade and Rita Zilh{\~a}o and Arraiano, {Cec{\'i}lia Maria}",

year = "1995",

month = apr,

day = "1",

doi = "10.1016/0378-1097(95)00060-I",

language = "English",

volume = "127",

pages = "187--193",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Federation of European Microbiological Societies | Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Construction and characterization of an absolute deletion mutant of Escherichia coli ribonuclease II

AU - Piedade, João

AU - Zilhão, Rita

AU - Arraiano, Cecília Maria

PY - 1995/4/1

Y1 - 1995/4/1

N2 - The degradation of mRNA plays a central role in the control of protein synthesis. In Escherichia coli, the rnb gene encodes ribonuclease II (RNase II), one of the two main exonucleases involved in mRNA decay. We have constructed strain CMA201, in which the rnb promoter region and the gene were deleted from the chromosome and replaced by a tettr cassette. This is the first rnb absolute deletion mutant that shows the complete absence of rnb-specific mRNA. This strain has growth characteristics similar to the wild-type, even though it has no RNase II activity, and it should be useful in studies of mRNA metabolism.

AB - The degradation of mRNA plays a central role in the control of protein synthesis. In Escherichia coli, the rnb gene encodes ribonuclease II (RNase II), one of the two main exonucleases involved in mRNA decay. We have constructed strain CMA201, in which the rnb promoter region and the gene were deleted from the chromosome and replaced by a tettr cassette. This is the first rnb absolute deletion mutant that shows the complete absence of rnb-specific mRNA. This strain has growth characteristics similar to the wild-type, even though it has no RNase II activity, and it should be useful in studies of mRNA metabolism.

KW - Deletion

KW - Escherichia coli

KW - mRNA degradation

KW - Ribonuclease II

KW - rnb

UR - http://www.scopus.com/inward/record.url?scp=0028987346&partnerID=8YFLogxK

UR - https://academic.oup.com/femsle/article/127/3/187/600766

U2 - 10.1016/0378-1097(95)00060-I

DO - 10.1016/0378-1097(95)00060-I

M3 - Article

C2 - 7758932

AN - SCOPUS:0028987346

SN - 0378-1097

VL - 127

SP - 187

EP - 193

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 3

ER -

Construction and characterization of an absolute deletion mutant of Escherichia coli ribonuclease II

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this