Conformational and orientational guidance of the analgesic dipeptide kyotorphin induced by lipidic membranes: Putative correlation toward receptor docking

Sílvia C.D.N. Lopes, Cláudio M. Soares, António M. Baptista, Erik Goormaghtigh, Benedito J.Costa Cabral, Miguel A.R.B. Castanho

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The analgesic dipeptide kyotorphin (L-Tyr-L-Arg) and an acylated kyotorphin derivative were studied by a combination of theoretical (molecular dynamics simulation and quantum mechanics methods) and experimental (fluorescence and infrared spectroscopies) approaches both in solution and in model systems of membranes. At biological pH the peptides have a neutral net charge. Nevertheless, their phenolic rings interact with phospholipid molecules (partition coefficient varies from 6 × 102 to 2 × 10 4, depending on the lipid and pH used) despite being exposed to the aqueous bulk medium. The lowest energy transition dipole moment is displaced from the normal to the lipid bilayer by 20° on average. The observed extensive interaction, pKa, precise location, and well-defined orientation in membranes combined with the ability to discriminate rigid raftlike membrane domains suggest that kyotorphin meets the structural constraints needed for receptor-ligand interaction. The acylated kyotorphin derivative mimics kyotorphin properties and represents a promising way for entrapment in a drug carrier and transport across the blood-brain barrier.

Original languageEnglish
Pages (from-to)3385-3394
Number of pages10
JournalJournal of Physical Chemistry B
Volume110
Issue number7
DOIs
Publication statusPublished - 23 Feb 2006

Keywords

  • Quantum theory
  • Solutions
  • Biological membranes
  • Blood
  • Brain
  • Computer simulation
  • Conformations
  • Derivatives
  • Infrared spectroscopy
  • Molecular dynamics
  • pH effects
  • Phospholipids
  • Proteins

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