Comprehensive analysis of the isomiRome in the vegetative organs of the conifer Pinus pinaster under contrasting water availability

Pedro Perdiguero, Andreia Santos Rodrigues, Inês Chaves, Bruno Costa, Ana Alves, Nuria de María, María Dolores Vélez, Carmen Díaz-Sala, María Teresa Cervera, Célia Maria Miguel

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

An increasing number of microRNAs (miRNAs) and miRNA-related sequences produced during miRNA biogenesis, comprising the isomiRome, have been recently highlighted in different species as critical mediators of environmental stress responses. Conifers have some of the largest known genomes but an extensive characterization of the isomiRome from any conifer species has been lacking. We provide here a comprehensive overview of the Pinus pinaster isomiRome expressed in roots, stem and needles under well-watered and drought conditions. From the 13,441 unique small RNA sequences identified, 2,980 were annotated as canonical miRNAs or miRNA* and the remaining were classified as isomiRNA or miRNA-like sequences. A survey of their expression patterns highlighted roots as the most responsive organ under drought, where specific sequences of which a 24-nt novel miRNA stood out, were strongly down-regulated. Given the putative roles of the miRNA-targeted transcripts validated specifically in root tissues, some of the miRNAs, conserved and novel, are shortlisted as potential regulators of drought response. These results provide a valuable resource for comparative studies between gymnosperms and angiosperms. Furthermore, it evidences high transferability of the isomiRome between pine species being a useful basis for further molecular regulation and physiological studies, and especially those focused on adaptation to drought conditions.

Original languageEnglish
Pages (from-to)706-728
Number of pages23
JournalPlant Cell and Environment
Volume44
Issue number3
DOIs
Publication statusPublished - Mar 2021

Keywords

  • isomiR
  • miRNA
  • organ specificity
  • pine
  • target genes
  • water stress

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