Aim: To gain detailed insight into the genetic organization of two multidrug resistance plasmids, pSM31 and pSM39, from clinical S. aureus isolates collected in a hospital in Lisbon, Portugal, and to identify factors that may explain their selection and persistence. Methods: Plasmid pSM31 was sequenced by Sanger sequencing using cloning and primer walking whereas pSM39 was sequenced by next generation sequencing. Sequence analysis was carried out using the BlastN and BlastP programs and the ORF Finder software from NCBI.Results: The two plasmids fully characterized in this work were non-conjugative multiresistance plas-mids. pSM31 (27,424 bp) is closely related to the family of globally dispersed plasmids from the pGSA23 group harbouring determinants for resistance to β-lactams and cadmium as well several enterotoxin genes. pSM39 (26,036 bp) is a newly described member of the pGSA11 group that carries genes con-ferring resistance to β-lactams, biocides, cadmium, zinc and mercury and comprises a region that may have been acquired by recombination with a S. epidermidis plasmid. Conclusions: The sequence analysis of these two plasmids confirmed their involvement in antimicrobial resistance among S. aureus strains isolated from a clinical setting. It also provided genetic evidence for the potential risk of co-selection of antibiotic resistance genes and/or virulence genes by selective pressure of antibiotics, biocides and/or heavy metals.
|Publication status||Published - 23 Aug 2018|
|Event||18th International Symposium on Staphylococci and Staphylococcal Infections - Copenhagen, Denmark|
Duration: 23 Aug 2018 → 26 Aug 2018
|Conference||18th International Symposium on Staphylococci and Staphylococcal Infections|
|Abbreviated title||18th ISSSI|
|Period||23/08/18 → 26/08/18|