Competitive counterion complexation allows the true host: guest binding constants from a single titration by ionic receptors

Márcia Pessêgo, Nuno Basílio, M. Carmen Muñiz, Luis García-Río

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Counterion competitive complexation is a background process currently ignored by using ionic hosts. Consequently, guest binding constants are strongly affected by the design of the titration experiments in such a way that the results are dependent on the guest concentration and on the presence of added salts, usually buffers. In the present manuscript we show that these experimental difficulties can be overcome by just considering the counterion competitive complexation. Moreover a single titration allows us to obtain not only the true binding constants but also the stoichiometry of the complex showing the formation of 1:1:1 (host:guest:counterion) complexes. The detection of high stoichiometry complexes is not restricted to a single titration experiment but also to a displacement assay where both competitive and competitive-cooperative complexation models are taken into consideration.

Original languageEnglish
Pages (from-to)6442-6448
Number of pages7
JournalOrganic & Biomolecular Chemistry
Volume14
Issue number27
DOIs
Publication statusPublished - 2016

Keywords

  • AQUEOUS-SOLUTION
  • P-SULFONATOCALIXARENES
  • HOST/GUEST COMPLEXES
  • ALPHA-CYCLODEXTRIN
  • METAL-CATIONS
  • NMR
  • MICROCALORIMETRY
  • SURFACTANTS
  • INCLUSION
  • BEHAVIORS

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