Mass spectrometry is extremely efficient for sequencing small peptides generated by, for example, a trypsin digestion of a complex mixture. Current instruments have the capacity to generate 50-100 K MSMS spectra from a single run. Of these ~30-50% is typically assigned to peptide matches on a 1% FDR threshold. The remaining spectra need more research to explain. We address here whether the 30-50% matched spectra provide consensus matches when using different database-dependent search pipelines. Although the majority of the spectra peptide assignments concur across search engines, our conclusion is that database-dependent search engines still require improvements.
- Database dependent search
- Peptide assignments