Comparative estrogenic responses of mirror carp (Cyprinus carpio) exposed to a treated municipal sewage effluent (Lisbon) in two seasonal periods.

Mário Emanuel Campos de Sousa Diniz, Isabel Peres, Jean Claude Pihan

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37 Citations (Scopus)

Abstract

To assess the estrogenic potency of the treated domestic sewage effluent from a sewage treatment plant (STP) located in Lisbon (Chelas), 60 mirror carp (Cyprinus carpio) were exposed to different concentrations of the sewage effluent (0%, 25%, 50% and 100%) for two periods of 28 days in two different seasons (winter/spring). Vitellogenin induction in males was used as a biomarker of exposure to xenoestrogens. At the end of the experiment, blood samples were taken for vitellogenin analysis and the fish were sacrificed and dissected. Gonad samples were taken for histological evaluation of the sewage effects. The results showed an increase in vitellogenin induction in exposed fish, both males and females, depending on the different dilutions of the sewage effluent. In comparison with controls, the gonadosomatic index decreased significantly (P<0.05) in fish exposed to 100% treated effluent. Although statistically not significant, the hepatosomatic index (HSI) was high in all exposed fish. Histological abnormalities in fish gonads were evaluated and related to the different percentages of sewage effluent. Seasonal variations found in estrogenic responses were attributed to weather influences on sewage dilution.
Original languageEnglish
Pages (from-to)129-139
Number of pages11
JournalThe Science of the total environment
Volume349
Issue numberNA
DOIs
Publication statusPublished - 15 Oct 2005

Fingerprint

Sewage
Effluents
Mirrors
sewage
Fish
effluent
Vitellogenins
fish
Dilution
dilution
Sewage treatment plants
Biomarkers
abnormality
sewage treatment
biomarker
Blood
seasonal variation
blood
weather
winter

Keywords

  • Sewage effluent
  • Vitellogenin
  • Cyprinus carpio
  • Endocrine disruption
  • Seasonal variations

Cite this

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title = "Comparative estrogenic responses of mirror carp (Cyprinus carpio) exposed to a treated municipal sewage effluent (Lisbon) in two seasonal periods.",
abstract = "To assess the estrogenic potency of the treated domestic sewage effluent from a sewage treatment plant (STP) located in Lisbon (Chelas), 60 mirror carp (Cyprinus carpio) were exposed to different concentrations of the sewage effluent (0{\%}, 25{\%}, 50{\%} and 100{\%}) for two periods of 28 days in two different seasons (winter/spring). Vitellogenin induction in males was used as a biomarker of exposure to xenoestrogens. At the end of the experiment, blood samples were taken for vitellogenin analysis and the fish were sacrificed and dissected. Gonad samples were taken for histological evaluation of the sewage effects. The results showed an increase in vitellogenin induction in exposed fish, both males and females, depending on the different dilutions of the sewage effluent. In comparison with controls, the gonadosomatic index decreased significantly (P<0.05) in fish exposed to 100{\%} treated effluent. Although statistically not significant, the hepatosomatic index (HSI) was high in all exposed fish. Histological abnormalities in fish gonads were evaluated and related to the different percentages of sewage effluent. Seasonal variations found in estrogenic responses were attributed to weather influences on sewage dilution.",
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AU - Pihan, Jean Claude

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