TY - JOUR
T1 - Common bean SNP alleles and candidate genes affecting photosynthesis under contrasting water regimes
AU - Leitão, Susana Trindade
AU - Bicho, Maria Catarina
AU - Pereira, Priscila
AU - Paulo, Maria João
AU - Malosetti, Marcos
AU - Araújo, Susana de Sousa
AU - van Eeuwijk, Fred
AU - Vaz Patto, Maria Carlota
N1 - Funding Information:
The authors are grateful to Steve Beebe, CIAT Genetic Resources Unit, Cali, Colombia, for providing seeds from the common bean lines SER16 and Tio Canela-75. We also acknowledge Maria Manuela Veloso, Research Unit of Biotechnology and Genetic Resources, INIAV, Oeiras, Portugal, for the multiplication of the Portuguese common bean collection. This work was supported by Fundação para a Ciência e Tecnologia (FCT), Portugal, through grant SFRH/BD/92160/2013 (S.T.L.), IF/01337/2014 FCT Investigator contract (M. C.V.P.), PhD holder contract DL57 (S.S.A.), research project BeGeQA (PTDC/AGR-TEC/3555/2012), and R&D Unit, UIDB/04551/2020 (GREEN-IT—Bioresources for Sustainability). European Union through COST Action FA1306, and PDR2020-784-042734 project are also acknowledged.
Publisher Copyright:
© 2020, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Water deficit is a major worldwide constraint to common bean (Phaseolus vulgaris L.) production, being photosynthesis one of the most affected physiological processes. To gain insights into the genetic basis of the photosynthetic response of common bean under water-limited conditions, a collection of 158 Portuguese accessions was grown under both well-watered and water-deficit regimes. Leaf gas-exchange parameters were measured and photosynthetic pigments quantified. The same collection was genotyped using SNP arrays, and SNP-trait associations tested considering a linear mixed model accounting for the genetic relatedness among accessions. A total of 133 SNP-trait associations were identified for net CO2 assimilation rate, transpiration rate, stomatal conductance, and chlorophylls a and b, carotenes, and xanthophyll contents. Ninety of these associations were detected under water-deficit and 43 under well-watered conditions, with only two associations common to both treatments. Identified candidate genes revealed that stomatal regulation, protein translocation across membranes, redox mechanisms, hormone, and osmotic stress signaling were the most relevant processes involved in common bean response to water-limited conditions. These candidates are now preferential targets for common bean water-deficit-tolerance breeding. Additionally, new sources of water-deficit tolerance of Andean, Mesoamerican, and admixed origin were detected as accessions valuable for breeding, and not yet explored.
AB - Water deficit is a major worldwide constraint to common bean (Phaseolus vulgaris L.) production, being photosynthesis one of the most affected physiological processes. To gain insights into the genetic basis of the photosynthetic response of common bean under water-limited conditions, a collection of 158 Portuguese accessions was grown under both well-watered and water-deficit regimes. Leaf gas-exchange parameters were measured and photosynthetic pigments quantified. The same collection was genotyped using SNP arrays, and SNP-trait associations tested considering a linear mixed model accounting for the genetic relatedness among accessions. A total of 133 SNP-trait associations were identified for net CO2 assimilation rate, transpiration rate, stomatal conductance, and chlorophylls a and b, carotenes, and xanthophyll contents. Ninety of these associations were detected under water-deficit and 43 under well-watered conditions, with only two associations common to both treatments. Identified candidate genes revealed that stomatal regulation, protein translocation across membranes, redox mechanisms, hormone, and osmotic stress signaling were the most relevant processes involved in common bean response to water-limited conditions. These candidates are now preferential targets for common bean water-deficit-tolerance breeding. Additionally, new sources of water-deficit tolerance of Andean, Mesoamerican, and admixed origin were detected as accessions valuable for breeding, and not yet explored.
UR - http://www.scopus.com/inward/record.url?scp=85098492468&partnerID=8YFLogxK
U2 - 10.1038/s41438-020-00434-6
DO - 10.1038/s41438-020-00434-6
M3 - Article
AN - SCOPUS:85098492468
SN - 2052-7276
VL - 8
JO - Horticulture Research
JF - Horticulture Research
IS - 1
M1 - 4
ER -