Combined proteomic and metallomic analyses in Scrobicularia plana clams to assess environmental pollution of estuarine ecosystems

Raul Gonzalez-Dominguez, Hugo Miguel Santos, Maria João Bebianno, Tamara Garcia-Barrera, José Luís Gomez-Ariza, José Luis Capelo

Research output: Contribution to journalArticle

Abstract

Estuaries are very important ecosystems with great ecological and economic value, but usually highly impacted by anthropogenic pressure. Thus, the assessment of pollution levels in these habitats is critical in order to evaluate their environmental quality. In this work, we combined complementary metallomic and proteomic approaches with the aim to monitor the effects of environmental pollution on Scrobicularia plana clams captured in three estuarine systems from the south coast of Portugal; Arade estuary, Ria Formosa and Guadiana estuary. Multi-elemental profiling of digestive glands was carried out to evaluate the differential pollution levels in the three study areas. Then, proteomic analysis by means of two-dimensional gel electrophoresis and mass spectrometry revealed twenty-one differential proteins, which could be associated with multiple toxicological mechanisms induced in environmentally stressed organisms. Accordingly, it could be concluded that the combination of different omic approaches presents a great potential in environmental research.

Original languageEnglish
Pages (from-to)117-124
Number of pages8
JournalMarine Pollution Bulletin
Volume113
Issue number1-2
DOIs
Publication statusPublished - 15 Dec 2016

Fingerprint

estuarine ecosystem
Aquatic ecosystems
proteomics
Estuaries
clams
Pollution
estuaries
pollution
estuary
ecosystems
ria
ecological value
environmental research
two-dimensional gel electrophoresis
environmental quality
Electrophoresis
economic valuation
Ecosystems
Portugal
Mass spectrometry

Keywords

  • Environmental pollution
  • Estuaries
  • Metallomics
  • Proteomics
  • Scrobicularia plana

Cite this

Gonzalez-Dominguez, Raul ; Santos, Hugo Miguel ; Bebianno, Maria João ; Garcia-Barrera, Tamara ; Gomez-Ariza, José Luís ; Capelo, José Luis. / Combined proteomic and metallomic analyses in Scrobicularia plana clams to assess environmental pollution of estuarine ecosystems. In: Marine Pollution Bulletin. 2016 ; Vol. 113, No. 1-2. pp. 117-124.
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abstract = "Estuaries are very important ecosystems with great ecological and economic value, but usually highly impacted by anthropogenic pressure. Thus, the assessment of pollution levels in these habitats is critical in order to evaluate their environmental quality. In this work, we combined complementary metallomic and proteomic approaches with the aim to monitor the effects of environmental pollution on Scrobicularia plana clams captured in three estuarine systems from the south coast of Portugal; Arade estuary, Ria Formosa and Guadiana estuary. Multi-elemental profiling of digestive glands was carried out to evaluate the differential pollution levels in the three study areas. Then, proteomic analysis by means of two-dimensional gel electrophoresis and mass spectrometry revealed twenty-one differential proteins, which could be associated with multiple toxicological mechanisms induced in environmentally stressed organisms. Accordingly, it could be concluded that the combination of different omic approaches presents a great potential in environmental research.",
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note = "Sem PDF conforme despacho. info:eu-repo/grantAgreement/FCT/5876/147218/PT# info:eu-repo/grantAgreement/FCT/5876/147258/PT# Dr. Raul Gonzalez-Dominguez thanks the Campus de Excelencia Internacional del Mar. (CEIMAR) for providing the financial support for the realization of a research stay in the framework of the mobility call CEIMAR (2014/2015) for pre-doctoral students from the University of Huelva. Dr. Hugo Santos and Dr. Jose Luis Capelo acknowledge support received from ProteoMass Scientific Society. Dr. Hugo Santos also acknowledges financial support provided by Fundarcao para a Ciencia e a Tecnologia, Ministerio da Ciencia, Tecnologia e Ensino Superior (FCT, Portugal) under grant SRFH/BPD/73997/2010. Dr. Capelo thanks the support given by (i) the Associate Laboratory for Green Chemistry LAQV, which is financed by national funds from FCT/MEC (UID/QUI/50006/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007265); and by (ii) the Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO which is financed by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). Dr. Garcia-Barrera and Dr. Gomez-Aria acknowledge financial support provided by the projects CTM2012-38720-C03-01 and CTM2015-67902-C2-1-P from the Spanish Ministry of Economy and Competitiveness and P12-FQM-0442 from the Regional Ministry of Economy, Innovation, Science and Employment (Andalusian Government, Spain). Finally, authors are grateful to FEDER (European Community) for financial support, grant number UNHU13-1E-1611.",
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Combined proteomic and metallomic analyses in Scrobicularia plana clams to assess environmental pollution of estuarine ecosystems. / Gonzalez-Dominguez, Raul; Santos, Hugo Miguel; Bebianno, Maria João; Garcia-Barrera, Tamara; Gomez-Ariza, José Luís ; Capelo, José Luis.

In: Marine Pollution Bulletin, Vol. 113, No. 1-2, 15.12.2016, p. 117-124.

Research output: Contribution to journalArticle

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