Combined molecular diagnosis of b-cell lymphomas with t(11;14)(q13;q32) or t(14;18)(q32;q21) using multiplex- and long distance inverse-polymerase chain reaction

Luís Vieira, Ana Martinho, Ofélia Antunes, Elizabeth Silva, Ana P. Ambrósio, Maria C. Geraldes, Rute Nascimento, Cĝndido Silva, José M. Pereira, Esmeraldina C. Júnior, Peter Jordan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Translocations t(14;18)(q32;q21) and t(11;14)(q13;q32) are recurrent findings in follicular lymphoma (FL) and mantle cell lymphoma (MCL), respectively. However, the molecular counterparts of these translocations can only be detected in up to 75% of FL and 50% of MCL cases using routine techniques. To improve the efficiency of detection, we first devised a single-tube multiplex-polymerase chain reaction (PCR) assay with primers located within a conserved immunoglobulin heavy chain (IGH) sequence and 5′ to the main breakpoint cluster regions of BCL2 and CCND1. Using this assay in 17 FL and 11 MCL diagnostic DNA samples, we readily identified a BCL2-IGH fusion in 65% of FL patients and a CCND1-IGH fusion in 55% of MCL patients. In the remaining cases, we used long distance inverse-PCR to detect BCL2-IGH and CCND1-IGH fusion genes with different BCL2 and CCND1 breakpoint locations. We found additional translocations in 3 patients (17%) with FL and in 4 patients (36%) with MCL. Taken together, we show that multiplex-PCR combined with long distance inverse-PCR detected a t(14;18) in 82% of FL patients and a t(11;14) in 91% of MCL patients, demonstrating that this 2-step protocol is an effective approach for molecular detection of t(11;14) and t(14;18) in B-cell lymphomas.

Original languageEnglish
Pages (from-to)73-81
Number of pages9
JournalDiagnostic Molecular Pathology
Volume17
Issue number2
DOIs
Publication statusPublished - 1 Jun 2008

Keywords

  • BCL2
  • CCND1
  • IGH
  • Long distance inverse-PCR
  • Multiplex-PCR

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