TY - JOUR
T1 - Combined hydrothermal pre-treatment and enzymatic hydrolysis of corn fibre
T2 - Production of ferulic acid extracts and assessment of their antioxidant and antiproliferative properties
AU - Valério, Rita
AU - Serra, Ana Teresa
AU - Baixinho, João
AU - Cardeira, Martim
AU - Fernández, Naiara
AU - Bronze, Maria Rosário
AU - Duarte, Luís C.
AU - Tavares, Maria L.
AU - Crespo, João G.
AU - Brazinha, Carla
N1 - Funding Information:
info:eu-repo/grantAgreement/FCT/5876/147218/PT#
FCT/MEC, Portugal , is acknowledged for the PhD Fellow grants SFRH/BD/138011/2018 in a PhD project within an industrial environment and Doctoral Program in Refining, Petrochemical and Chemical Engineering (EngIQ).
The company Copam - Companhia Portuguesa De Amidos, S.A (Portugal) is also acknowledge for the financial support through the PhD Fellow grant PD/BDE/113543/2015.
This work was supported by the Applied Molecular Biosciences Unit- UCIBIO which is financed by national funds from FCT/MCTES (UID/Multi/04378/2019) and the Associate Laboratory for Green Chemistry- LAQV and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER – 007265). iNOVA4Health – UIDB/04462/2020 and UIDP/04462/2020, a program financially supported by Fundação para a Ciência e Tecnologia / Ministério da Ciência, Tecnologia e Ensino Superior , through national funds is acknowledged. Funding from INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC), is gratefully acknowledged. ATS also acknowledge Fundação para a Ciência e Tecnologia/Ministério da Educação e Ciência for the Individual Grant CEECIND/04801/2017. Univar Solution company is also acknowledged for providing the commercial preparation enzymes from Novozymes A/S.
PY - 2021/10/15
Y1 - 2021/10/15
N2 - Ferulic acid may be used as a nutraceutical ingredient or as a substrate to produce bio-vanillin. There is an increasing market demand for ferulic acid obtained from natural sources such as low-cost agro-industrial by-products, due to its potential applications as nutraceutical ingredient and as a substrate to produce bio-vanillin. This work aims to study ferulic acid recovery from corn fibre (one of the most abundant natural sources of ferulic acid), involving an integrated process of hydrothermal pre-treatment followed by enzymatic hydrolysis. The objective is primarily to produce natural extracts with a maximum ferulic acid recovery yield, but it is also to assess their antioxidant and antiproliferative properties and their cytotoxicity. Different commercial enzyme preparations were tested for release of ferulic acid from corn fibre. The best results were obtained for Ultraflo ®XL in a concentration of 2 % (wenzyme preparation/w dry corn fibre) at a pH of 5 and at 55 °C, presenting a recovery yield of esterified ferulic acid of 7.83 ± 1.35 % (wrecovered ferulic acid/wtotal esterified ferulic acid), which corresponds to 0.13 ± 0.02 % (wferulic acid/w dry corn fibre). When using a hydrothermal pre-treatment at a temperature of 140 °C for 40 min, prior to the use of the same enzymatic hydrolysis procedure, the recovery yield of esterified ferulic acid increased to 28.94 ± 2.40 % (w recovered ferulic acid/ wtotal esterified ferulic acid), which corresponds to 4.9 ± 0.3 % (wferulic acid/wdry corn fibre). The use of this pre-treatment leads not only to the highest yield of ferulic acid, but also to the lowest concentration of furfural and hydroxymethylfurfural, without the formation of formic and levulinic acid (not detected). All pre-treatments tested led to an improved quality of the extract in terms of bioactivity.
AB - Ferulic acid may be used as a nutraceutical ingredient or as a substrate to produce bio-vanillin. There is an increasing market demand for ferulic acid obtained from natural sources such as low-cost agro-industrial by-products, due to its potential applications as nutraceutical ingredient and as a substrate to produce bio-vanillin. This work aims to study ferulic acid recovery from corn fibre (one of the most abundant natural sources of ferulic acid), involving an integrated process of hydrothermal pre-treatment followed by enzymatic hydrolysis. The objective is primarily to produce natural extracts with a maximum ferulic acid recovery yield, but it is also to assess their antioxidant and antiproliferative properties and their cytotoxicity. Different commercial enzyme preparations were tested for release of ferulic acid from corn fibre. The best results were obtained for Ultraflo ®XL in a concentration of 2 % (wenzyme preparation/w dry corn fibre) at a pH of 5 and at 55 °C, presenting a recovery yield of esterified ferulic acid of 7.83 ± 1.35 % (wrecovered ferulic acid/wtotal esterified ferulic acid), which corresponds to 0.13 ± 0.02 % (wferulic acid/w dry corn fibre). When using a hydrothermal pre-treatment at a temperature of 140 °C for 40 min, prior to the use of the same enzymatic hydrolysis procedure, the recovery yield of esterified ferulic acid increased to 28.94 ± 2.40 % (w recovered ferulic acid/ wtotal esterified ferulic acid), which corresponds to 4.9 ± 0.3 % (wferulic acid/wdry corn fibre). The use of this pre-treatment leads not only to the highest yield of ferulic acid, but also to the lowest concentration of furfural and hydroxymethylfurfural, without the formation of formic and levulinic acid (not detected). All pre-treatments tested led to an improved quality of the extract in terms of bioactivity.
KW - Corn fibre
KW - Enzymatic hydrolysis
KW - Ferulic acid
KW - Hydrothermal pre-treatment
UR - http://www.scopus.com/inward/record.url?scp=85107761862&partnerID=8YFLogxK
U2 - 10.1016/j.indcrop.2021.113731
DO - 10.1016/j.indcrop.2021.113731
M3 - Article
AN - SCOPUS:85107761862
SN - 0926-6690
VL - 170
JO - Industrial Crops and Products
JF - Industrial Crops and Products
M1 - 113731
ER -