TY - PAT
T1 - Colorimetric method and kit for the detection of specific nucleic acid sequences using metal nanoparticles functionalized with modified oligonucleotides
AU - Tavares, Jose Ricardo Ramos Franco
AU - Baptista , Pedro Miguel Ribeiro Viana
AU - Doria , Goncalo Maria Reimão Pinto De Franca
AU - Flores, Alcino Orfeu de Leão
PY - 2010/3/25
Y1 - 2010/3/25
N2 - The present invention relates to a colorimetric method for the detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences, through the aggregation of nanoparticles functionalized with modified oligonucleotides, induced by an increase of the medium's ionic strength. Another aspect of the present invention relates with the development of a kit based on the method of the present invention, allowing for a quick and easy detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences.
AB - The present invention relates to a colorimetric method for the detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences, through the aggregation of nanoparticles functionalized with modified oligonucleotides, induced by an increase of the medium's ionic strength. Another aspect of the present invention relates with the development of a kit based on the method of the present invention, allowing for a quick and easy detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences.
M3 - Regional/Other National Application
M1 - US2010075335
Y2 - 2009/11/04
ER -