Colorimetric method and kit for the detection of specific nucleic acid sequences using metal nanoparticles functionalized with modified oligonucleotides

Jose Ricardo Ramos Franco Tavares (Inventor), Pedro Miguel Ribeiro Viana Baptista (Inventor), Goncalo Maria Reimao Pinto De Franca Doria (Inventor), Alcino Orfeu De Leao Flores (Inventor)

Research output: PatentRegional/Other National Application

Abstract

The present invention relates to a colorimetric method for the detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences, through the aggregation of nanoparticles functionalized with modified oligonucleotides, induced by an increase of the medium's ionic strength. Another aspect of the present invention relates with the development of a kit based on the method of the present invention, allowing for a quick and easy detection of specific nucleic acids sequences, including mutations or single nucleotide polymorphisms within nucleic acid sequences.
Original languageEnglish
Patent numberEP2145023
IPCC12Q 1/ 68 A I
Priority date4/05/07
Filing date17/11/09
Publication statusPublished - 20 Jan 2010

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