Colorimetric detection of eukaryotic gene expression with DNA-derivatized gold nanoparticles

Pedro Miguel Ribeiro Viana Baptista, Gonçalo Dória, David Henriques, Eulália Pereira, Ricardo Franco

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Thiol-linked DNA-gold nanoparticles were used in a novel colorimetric method to detect the presence of specific mRNA from a total RNA extract of yeast cells. The method allowed detection of expression of the FSY1 gene that encodes a specific fructose/H+ symporter in Saccharomyces bayanus PYCC 4565. FSY1 is strongly expressed when the yeast is grown in fructose as the sole carbon source, while cells cultivated in glucose as the sole carbon source repress gene expression. The presence of FSY1 mRNA is detected based on color change of a sample containing total RNA extracted from the organism and gold nanoparticles derivatized with a 15-mer of complementary single stranded DNA upon addition of NaCl. If FSY1 mRNA is present, the solution remains pink, changing to blue-purple in the absence of FSY1 mRNA. Direct detection of specific expression was possible from only 0.3 microg of unamplified total RNA without any further enhancement. This novel method is inexpensive, very easy to perform as no amplification or signal enhancement steps are necessary and takes less than 15 min to develop after total RNA extraction. No temperature control is necessary and color change can be easily detected visually.
Original languageEnglish
Pages (from-to)111-7
Number of pages7
JournalJournal of Biotechnology
Volume119
Issue number2
DOIs
Publication statusPublished - 1 Jan 2005

Fingerprint

RNA
Gene expression
Gold
Nanoparticles
DNA
Gene Expression
Messenger RNA
Fructose
Yeast
Carbon
Color
Yeasts
Symporters
Saccharomyces
Single-Stranded DNA
Cell Extracts
Sulfhydryl Compounds
Temperature control
Amplification
Glucose

Keywords

  • Nanotechnology
  • RNA detection
  • Biosensors
  • Gene expression detection
  • Gold nanoparticles

Cite this

@article{5d7ab92067724b1fbd9c68ad3e0dc65c,
title = "Colorimetric detection of eukaryotic gene expression with DNA-derivatized gold nanoparticles",
abstract = "Thiol-linked DNA-gold nanoparticles were used in a novel colorimetric method to detect the presence of specific mRNA from a total RNA extract of yeast cells. The method allowed detection of expression of the FSY1 gene that encodes a specific fructose/H+ symporter in Saccharomyces bayanus PYCC 4565. FSY1 is strongly expressed when the yeast is grown in fructose as the sole carbon source, while cells cultivated in glucose as the sole carbon source repress gene expression. The presence of FSY1 mRNA is detected based on color change of a sample containing total RNA extracted from the organism and gold nanoparticles derivatized with a 15-mer of complementary single stranded DNA upon addition of NaCl. If FSY1 mRNA is present, the solution remains pink, changing to blue-purple in the absence of FSY1 mRNA. Direct detection of specific expression was possible from only 0.3 microg of unamplified total RNA without any further enhancement. This novel method is inexpensive, very easy to perform as no amplification or signal enhancement steps are necessary and takes less than 15 min to develop after total RNA extraction. No temperature control is necessary and color change can be easily detected visually.",
keywords = "Nanotechnology, RNA detection, Biosensors, Gene expression detection, Gold nanoparticles",
author = "Baptista, {Pedro Miguel Ribeiro Viana} and Gon{\cc}alo D{\'o}ria and David Henriques and Eul{\'a}lia Pereira and Ricardo Franco",
note = "Sem pdf.",
year = "2005",
month = "1",
day = "1",
doi = "10.1016/j.jbiotec.2005.02.019",
language = "English",
volume = "119",
pages = "111--7",
journal = "Journal of Biotechnology",
issn = "0168-1656",
publisher = "Elsevier Science B.V., Inc",
number = "2",

}

Colorimetric detection of eukaryotic gene expression with DNA-derivatized gold nanoparticles. / Baptista, Pedro Miguel Ribeiro Viana; Dória, Gonçalo; Henriques, David; Pereira, Eulália; Franco, Ricardo.

In: Journal of Biotechnology, Vol. 119, No. 2, 01.01.2005, p. 111-7.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Colorimetric detection of eukaryotic gene expression with DNA-derivatized gold nanoparticles

AU - Baptista, Pedro Miguel Ribeiro Viana

AU - Dória, Gonçalo

AU - Henriques, David

AU - Pereira, Eulália

AU - Franco, Ricardo

N1 - Sem pdf.

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Thiol-linked DNA-gold nanoparticles were used in a novel colorimetric method to detect the presence of specific mRNA from a total RNA extract of yeast cells. The method allowed detection of expression of the FSY1 gene that encodes a specific fructose/H+ symporter in Saccharomyces bayanus PYCC 4565. FSY1 is strongly expressed when the yeast is grown in fructose as the sole carbon source, while cells cultivated in glucose as the sole carbon source repress gene expression. The presence of FSY1 mRNA is detected based on color change of a sample containing total RNA extracted from the organism and gold nanoparticles derivatized with a 15-mer of complementary single stranded DNA upon addition of NaCl. If FSY1 mRNA is present, the solution remains pink, changing to blue-purple in the absence of FSY1 mRNA. Direct detection of specific expression was possible from only 0.3 microg of unamplified total RNA without any further enhancement. This novel method is inexpensive, very easy to perform as no amplification or signal enhancement steps are necessary and takes less than 15 min to develop after total RNA extraction. No temperature control is necessary and color change can be easily detected visually.

AB - Thiol-linked DNA-gold nanoparticles were used in a novel colorimetric method to detect the presence of specific mRNA from a total RNA extract of yeast cells. The method allowed detection of expression of the FSY1 gene that encodes a specific fructose/H+ symporter in Saccharomyces bayanus PYCC 4565. FSY1 is strongly expressed when the yeast is grown in fructose as the sole carbon source, while cells cultivated in glucose as the sole carbon source repress gene expression. The presence of FSY1 mRNA is detected based on color change of a sample containing total RNA extracted from the organism and gold nanoparticles derivatized with a 15-mer of complementary single stranded DNA upon addition of NaCl. If FSY1 mRNA is present, the solution remains pink, changing to blue-purple in the absence of FSY1 mRNA. Direct detection of specific expression was possible from only 0.3 microg of unamplified total RNA without any further enhancement. This novel method is inexpensive, very easy to perform as no amplification or signal enhancement steps are necessary and takes less than 15 min to develop after total RNA extraction. No temperature control is necessary and color change can be easily detected visually.

KW - Nanotechnology

KW - RNA detection

KW - Biosensors

KW - Gene expression detection

KW - Gold nanoparticles

U2 - 10.1016/j.jbiotec.2005.02.019

DO - 10.1016/j.jbiotec.2005.02.019

M3 - Article

VL - 119

SP - 111

EP - 117

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

IS - 2

ER -