Co-production of chitin-glucan complex and xylitol by Komagataella pastoris using glucose and xylose mixtures as carbon source

Diana Araújo, Filomena Freitas, Chantal Sevrin, Christian Grandfils, Maria A.M. Reis

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Komagataella pastoris was cultivated in glucose/xylose mixtures for production of chitin-glucan complex (CGC), a cell-wall polysaccharide. The culture preferred glucose as substrate for growth, resulting in high biomass yields (0.46–0.54 g/g). After glucose depletion, xylose was consumed but no cell growth was observed, indicating K. pastoris was unable to use it for growth. Interestingly, concomitant with xylose consumption, xylitol synthesis was noticed, reaching a maximum concentration of 7.64 g/L, with a yield on xylose of 0.52 g/g. Lower CGC production was reached as the xylose content was increased in the substrate mixtures, due to the lower biomass production. Moreover, cultivation in the presence of xylitol resulted in CGC enriched in chitin with higher molecular weight. These results suggest the possibility of using K. pastoris for the co-production of CGC and xylitol using glucose/xylose-rich substrates. It may also be a strategy to tailor CGC composition and average molecular weight.

Original languageEnglish
Pages (from-to)24-30
Number of pages7
JournalCarbohydrate Polymers
Volume166
DOIs
Publication statusPublished - 15 Jun 2017

Fingerprint

Xylitol
Chitin
Xylose
Glucans
Glucose
Carbon
Biomass
Substrates
Molecular weight
Cell growth
Polysaccharides
xylose-glucose
Chemical analysis

Keywords

  • Chitin-glucan complex (CGC)
  • Glucose
  • Komagataella pastoris
  • Xylitol
  • Xylose

Cite this

@article{aac353105ec24b67baf7d67c37bedd94,
title = "Co-production of chitin-glucan complex and xylitol by Komagataella pastoris using glucose and xylose mixtures as carbon source",
abstract = "Komagataella pastoris was cultivated in glucose/xylose mixtures for production of chitin-glucan complex (CGC), a cell-wall polysaccharide. The culture preferred glucose as substrate for growth, resulting in high biomass yields (0.46–0.54 g/g). After glucose depletion, xylose was consumed but no cell growth was observed, indicating K. pastoris was unable to use it for growth. Interestingly, concomitant with xylose consumption, xylitol synthesis was noticed, reaching a maximum concentration of 7.64 g/L, with a yield on xylose of 0.52 g/g. Lower CGC production was reached as the xylose content was increased in the substrate mixtures, due to the lower biomass production. Moreover, cultivation in the presence of xylitol resulted in CGC enriched in chitin with higher molecular weight. These results suggest the possibility of using K. pastoris for the co-production of CGC and xylitol using glucose/xylose-rich substrates. It may also be a strategy to tailor CGC composition and average molecular weight.",
keywords = "Chitin-glucan complex (CGC), Glucose, Komagataella pastoris, Xylitol, Xylose",
author = "Diana Ara{\'u}jo and Filomena Freitas and Chantal Sevrin and Christian Grandfils and Reis, {Maria A.M.}",
note = "This work was supported by the Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), which is financed by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF, under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). The authors acknowledge FCT/MEC, for fellowship SFRH/BPD/72280/2010.",
year = "2017",
month = "6",
day = "15",
doi = "10.1016/j.carbpol.2017.02.088",
language = "English",
volume = "166",
pages = "24--30",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "Elsevier Ltd",

}

Co-production of chitin-glucan complex and xylitol by Komagataella pastoris using glucose and xylose mixtures as carbon source. / Araújo, Diana; Freitas, Filomena; Sevrin, Chantal; Grandfils, Christian; Reis, Maria A.M.

In: Carbohydrate Polymers, Vol. 166, 15.06.2017, p. 24-30.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Co-production of chitin-glucan complex and xylitol by Komagataella pastoris using glucose and xylose mixtures as carbon source

AU - Araújo, Diana

AU - Freitas, Filomena

AU - Sevrin, Chantal

AU - Grandfils, Christian

AU - Reis, Maria A.M.

N1 - This work was supported by the Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), which is financed by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF, under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). The authors acknowledge FCT/MEC, for fellowship SFRH/BPD/72280/2010.

PY - 2017/6/15

Y1 - 2017/6/15

N2 - Komagataella pastoris was cultivated in glucose/xylose mixtures for production of chitin-glucan complex (CGC), a cell-wall polysaccharide. The culture preferred glucose as substrate for growth, resulting in high biomass yields (0.46–0.54 g/g). After glucose depletion, xylose was consumed but no cell growth was observed, indicating K. pastoris was unable to use it for growth. Interestingly, concomitant with xylose consumption, xylitol synthesis was noticed, reaching a maximum concentration of 7.64 g/L, with a yield on xylose of 0.52 g/g. Lower CGC production was reached as the xylose content was increased in the substrate mixtures, due to the lower biomass production. Moreover, cultivation in the presence of xylitol resulted in CGC enriched in chitin with higher molecular weight. These results suggest the possibility of using K. pastoris for the co-production of CGC and xylitol using glucose/xylose-rich substrates. It may also be a strategy to tailor CGC composition and average molecular weight.

AB - Komagataella pastoris was cultivated in glucose/xylose mixtures for production of chitin-glucan complex (CGC), a cell-wall polysaccharide. The culture preferred glucose as substrate for growth, resulting in high biomass yields (0.46–0.54 g/g). After glucose depletion, xylose was consumed but no cell growth was observed, indicating K. pastoris was unable to use it for growth. Interestingly, concomitant with xylose consumption, xylitol synthesis was noticed, reaching a maximum concentration of 7.64 g/L, with a yield on xylose of 0.52 g/g. Lower CGC production was reached as the xylose content was increased in the substrate mixtures, due to the lower biomass production. Moreover, cultivation in the presence of xylitol resulted in CGC enriched in chitin with higher molecular weight. These results suggest the possibility of using K. pastoris for the co-production of CGC and xylitol using glucose/xylose-rich substrates. It may also be a strategy to tailor CGC composition and average molecular weight.

KW - Chitin-glucan complex (CGC)

KW - Glucose

KW - Komagataella pastoris

KW - Xylitol

KW - Xylose

UR - http://www.scopus.com/inward/record.url?scp=85014055779&partnerID=8YFLogxK

U2 - 10.1016/j.carbpol.2017.02.088

DO - 10.1016/j.carbpol.2017.02.088

M3 - Article

VL - 166

SP - 24

EP - 30

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

ER -