Cloning, expression, purification, crystallization and preliminary crystallographic studies of BceC, a UDP-glucose dehydrogenase from Burkholderia cepacia IST408

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Abstract

Bacteria of the Burkholderia cepacia complex (Bcc) have emerged as important opportunistic pathogens, establishing lung infections in immunocompromised or cystic fibrosis patients. Bcc uses polysaccharide-biofilm production in order to evade the host immune response. The biofilm precursor UDP-glucuronic acid is produced by a twofold NAD(+)-dependent oxidation of UDP-glucose. In B. cepacia IST408 this enzymatic reaction is performed by the UDP-glucose dehydrogenase BceC, a 470-residue enzyme, the production and crystallization of which are described here. The crystals belonged to the orthorhombic space group P2(1)2(1)2(1) and contained four molecules in the asymmetric unit. Their crystallographic analysis at 2.09 angstrom resolution and a molecular-replacement study are reported.
Original languageUnknown
Pages (from-to)269-271
JournalActa Crystallographica Section F-Structural Biology And Crystallization Com
Volume66
Issue numberPart 3
DOIs
Publication statusPublished - 1 Jan 2010

Cite this

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title = "Cloning, expression, purification, crystallization and preliminary crystallographic studies of BceC, a UDP-glucose dehydrogenase from Burkholderia cepacia IST408",
abstract = "Bacteria of the Burkholderia cepacia complex (Bcc) have emerged as important opportunistic pathogens, establishing lung infections in immunocompromised or cystic fibrosis patients. Bcc uses polysaccharide-biofilm production in order to evade the host immune response. The biofilm precursor UDP-glucuronic acid is produced by a twofold NAD(+)-dependent oxidation of UDP-glucose. In B. cepacia IST408 this enzymatic reaction is performed by the UDP-glucose dehydrogenase BceC, a 470-residue enzyme, the production and crystallization of which are described here. The crystals belonged to the orthorhombic space group P2(1)2(1)2(1) and contained four molecules in the asymmetric unit. Their crystallographic analysis at 2.09 angstrom resolution and a molecular-replacement study are reported.",
keywords = "EXOPOLYSACCHARIDE, COMPLEX, CRYSTALS",
author = "Frazao, {Carlos Maria}",
year = "2010",
month = "1",
day = "1",
doi = "10.1107/s1744309109053500",
language = "Unknown",
volume = "66",
pages = "269--271",
journal = "Acta Crystallographica Section F-Structural Biology And Crystallization Com",
issn = "1744-3091",
publisher = "Blackwell Publishing Ltd",
number = "Part 3",

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TY - JOUR

T1 - Cloning, expression, purification, crystallization and preliminary crystallographic studies of BceC, a UDP-glucose dehydrogenase from Burkholderia cepacia IST408

AU - Frazao, Carlos Maria

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Bacteria of the Burkholderia cepacia complex (Bcc) have emerged as important opportunistic pathogens, establishing lung infections in immunocompromised or cystic fibrosis patients. Bcc uses polysaccharide-biofilm production in order to evade the host immune response. The biofilm precursor UDP-glucuronic acid is produced by a twofold NAD(+)-dependent oxidation of UDP-glucose. In B. cepacia IST408 this enzymatic reaction is performed by the UDP-glucose dehydrogenase BceC, a 470-residue enzyme, the production and crystallization of which are described here. The crystals belonged to the orthorhombic space group P2(1)2(1)2(1) and contained four molecules in the asymmetric unit. Their crystallographic analysis at 2.09 angstrom resolution and a molecular-replacement study are reported.

AB - Bacteria of the Burkholderia cepacia complex (Bcc) have emerged as important opportunistic pathogens, establishing lung infections in immunocompromised or cystic fibrosis patients. Bcc uses polysaccharide-biofilm production in order to evade the host immune response. The biofilm precursor UDP-glucuronic acid is produced by a twofold NAD(+)-dependent oxidation of UDP-glucose. In B. cepacia IST408 this enzymatic reaction is performed by the UDP-glucose dehydrogenase BceC, a 470-residue enzyme, the production and crystallization of which are described here. The crystals belonged to the orthorhombic space group P2(1)2(1)2(1) and contained four molecules in the asymmetric unit. Their crystallographic analysis at 2.09 angstrom resolution and a molecular-replacement study are reported.

KW - EXOPOLYSACCHARIDE

KW - COMPLEX

KW - CRYSTALS

U2 - 10.1107/s1744309109053500

DO - 10.1107/s1744309109053500

M3 - Article

VL - 66

SP - 269

EP - 271

JO - Acta Crystallographica Section F-Structural Biology And Crystallization Com

JF - Acta Crystallographica Section F-Structural Biology And Crystallization Com

SN - 1744-3091

IS - Part 3

ER -