Cloning and characterization of two ubiquitin::79-amino-acid extension protein-encoding fusion genes from Lupinus albus

António Jacinto, Ana M. Neves, Tatiana D. Vassilevskaia, Candido P. Ricardo, Claudina Rodrigues-Pousada

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Two different ubiquitin::79-amino-acid (aa) extension protein-encoding fusion genes were isolated from a Lupinus albus nuclear DNA library and sequenced. Both genes have 465-nucleotide open reading frames encoding a single ubiquitin (Ub) monomer fused in frame to a 79-aa extension (Ext) protein. The deduced aa sequences of the encoded Ub are identical to the aa sequences of Ub from other plants. The encoded Ext proteins are putative ribosomal proteins, highly basic, differing by 2 aa from each other, and have a high degree of similarity to Ext proteins from other plants.

Original languageEnglish
Pages (from-to)201-205
Number of pages5
JournalGene
Volume139
Issue number2
DOIs
Publication statusPublished - 25 Feb 1994

Fingerprint

Lupinus
Gene Fusion
Ubiquitin
Organism Cloning
Amino Acids
Amino Acid Sequence
Proteins
Plant Proteins
Ribosomal Proteins
Gene Library
Open Reading Frames
Nucleotides
Genes

Keywords

  • codon usage
  • dicotyledons
  • Gene family
  • legumes
  • nuclear signal
  • ribosomal protein

Cite this

@article{484e843c2772474ab1ee938aabc570c2,
title = "Cloning and characterization of two ubiquitin::79-amino-acid extension protein-encoding fusion genes from Lupinus albus",
abstract = "Two different ubiquitin::79-amino-acid (aa) extension protein-encoding fusion genes were isolated from a Lupinus albus nuclear DNA library and sequenced. Both genes have 465-nucleotide open reading frames encoding a single ubiquitin (Ub) monomer fused in frame to a 79-aa extension (Ext) protein. The deduced aa sequences of the encoded Ub are identical to the aa sequences of Ub from other plants. The encoded Ext proteins are putative ribosomal proteins, highly basic, differing by 2 aa from each other, and have a high degree of similarity to Ext proteins from other plants.",
keywords = "codon usage, dicotyledons, Gene family, legumes, nuclear signal, ribosomal protein",
author = "Ant{\'o}nio Jacinto and Neves, {Ana M.} and Vassilevskaia, {Tatiana D.} and Ricardo, {Candido P.} and Claudina Rodrigues-Pousada",
year = "1994",
month = "2",
day = "25",
doi = "10.1016/0378-1119(94)90755-2",
language = "English",
volume = "139",
pages = "201--205",
journal = "Gene Therapy",
issn = "0969-7128",
publisher = "Nature Publishing Group",
number = "2",

}

Cloning and characterization of two ubiquitin::79-amino-acid extension protein-encoding fusion genes from Lupinus albus. / Jacinto, António; Neves, Ana M.; Vassilevskaia, Tatiana D.; Ricardo, Candido P.; Rodrigues-Pousada, Claudina.

In: Gene, Vol. 139, No. 2, 25.02.1994, p. 201-205.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cloning and characterization of two ubiquitin::79-amino-acid extension protein-encoding fusion genes from Lupinus albus

AU - Jacinto, António

AU - Neves, Ana M.

AU - Vassilevskaia, Tatiana D.

AU - Ricardo, Candido P.

AU - Rodrigues-Pousada, Claudina

PY - 1994/2/25

Y1 - 1994/2/25

N2 - Two different ubiquitin::79-amino-acid (aa) extension protein-encoding fusion genes were isolated from a Lupinus albus nuclear DNA library and sequenced. Both genes have 465-nucleotide open reading frames encoding a single ubiquitin (Ub) monomer fused in frame to a 79-aa extension (Ext) protein. The deduced aa sequences of the encoded Ub are identical to the aa sequences of Ub from other plants. The encoded Ext proteins are putative ribosomal proteins, highly basic, differing by 2 aa from each other, and have a high degree of similarity to Ext proteins from other plants.

AB - Two different ubiquitin::79-amino-acid (aa) extension protein-encoding fusion genes were isolated from a Lupinus albus nuclear DNA library and sequenced. Both genes have 465-nucleotide open reading frames encoding a single ubiquitin (Ub) monomer fused in frame to a 79-aa extension (Ext) protein. The deduced aa sequences of the encoded Ub are identical to the aa sequences of Ub from other plants. The encoded Ext proteins are putative ribosomal proteins, highly basic, differing by 2 aa from each other, and have a high degree of similarity to Ext proteins from other plants.

KW - codon usage

KW - dicotyledons

KW - Gene family

KW - legumes

KW - nuclear signal

KW - ribosomal protein

UR - http://www.scopus.com/inward/record.url?scp=0028300216&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(94)90755-2

DO - 10.1016/0378-1119(94)90755-2

M3 - Article

VL - 139

SP - 201

EP - 205

JO - Gene Therapy

JF - Gene Therapy

SN - 0969-7128

IS - 2

ER -