Cloning and characterization of two ubiquitin::79-amino-acid extension protein-encoding fusion genes from Lupinus albus

António Jacinto; Ana M. Neves; Tatiana D. Vassilevskaia; Candido P. Ricardo; Claudina Rodrigues-Pousada

doi:10.1016/0378-1119(94)90755-2

Cloning and characterization of two ubiquitin::79-amino-acid extension protein-encoding fusion genes from Lupinus albus

António Jacinto, Ana M. Neves, Tatiana D. Vassilevskaia, Candido P. Ricardo, Claudina Rodrigues-Pousada

Instituto de Tecnologia Química e Biológica António Xavier (ITQB)

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Two different ubiquitin::79-amino-acid (aa) extension protein-encoding fusion genes were isolated from a Lupinus albus nuclear DNA library and sequenced. Both genes have 465-nucleotide open reading frames encoding a single ubiquitin (Ub) monomer fused in frame to a 79-aa extension (Ext) protein. The deduced aa sequences of the encoded Ub are identical to the aa sequences of Ub from other plants. The encoded Ext proteins are putative ribosomal proteins, highly basic, differing by 2 aa from each other, and have a high degree of similarity to Ext proteins from other plants.

Original language	English
Pages (from-to)	201-205
Number of pages	5
Journal	Gene
Volume	139
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(94)90755-2
Publication status	Published - 25 Feb 1994

Keywords

codon usage
dicotyledons
Gene family
legumes
nuclear signal
ribosomal protein

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10.1016/0378-1119(94)90755-2

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title = "Cloning and characterization of two ubiquitin::79-amino-acid extension protein-encoding fusion genes from Lupinus albus",

abstract = "Two different ubiquitin::79-amino-acid (aa) extension protein-encoding fusion genes were isolated from a Lupinus albus nuclear DNA library and sequenced. Both genes have 465-nucleotide open reading frames encoding a single ubiquitin (Ub) monomer fused in frame to a 79-aa extension (Ext) protein. The deduced aa sequences of the encoded Ub are identical to the aa sequences of Ub from other plants. The encoded Ext proteins are putative ribosomal proteins, highly basic, differing by 2 aa from each other, and have a high degree of similarity to Ext proteins from other plants.",

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author = "Ant{\'o}nio Jacinto and Neves, {Ana M.} and Vassilevskaia, {Tatiana D.} and Ricardo, {Candido P.} and Claudina Rodrigues-Pousada",

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T1 - Cloning and characterization of two ubiquitin::79-amino-acid extension protein-encoding fusion genes from Lupinus albus

AU - Jacinto, António

AU - Neves, Ana M.

AU - Vassilevskaia, Tatiana D.

AU - Ricardo, Candido P.

AU - Rodrigues-Pousada, Claudina

PY - 1994/2/25

Y1 - 1994/2/25

N2 - Two different ubiquitin::79-amino-acid (aa) extension protein-encoding fusion genes were isolated from a Lupinus albus nuclear DNA library and sequenced. Both genes have 465-nucleotide open reading frames encoding a single ubiquitin (Ub) monomer fused in frame to a 79-aa extension (Ext) protein. The deduced aa sequences of the encoded Ub are identical to the aa sequences of Ub from other plants. The encoded Ext proteins are putative ribosomal proteins, highly basic, differing by 2 aa from each other, and have a high degree of similarity to Ext proteins from other plants.

AB - Two different ubiquitin::79-amino-acid (aa) extension protein-encoding fusion genes were isolated from a Lupinus albus nuclear DNA library and sequenced. Both genes have 465-nucleotide open reading frames encoding a single ubiquitin (Ub) monomer fused in frame to a 79-aa extension (Ext) protein. The deduced aa sequences of the encoded Ub are identical to the aa sequences of Ub from other plants. The encoded Ext proteins are putative ribosomal proteins, highly basic, differing by 2 aa from each other, and have a high degree of similarity to Ext proteins from other plants.

KW - codon usage

KW - dicotyledons

KW - Gene family

KW - legumes

KW - nuclear signal

KW - ribosomal protein

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ER -

Cloning and characterization of two ubiquitin::79-amino-acid extension protein-encoding fusion genes from Lupinus albus

Abstract

Keywords

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