Cloning and characterization of two ubiquitin::79-amino-acid extension protein-encoding fusion genes from Lupinus albus

António Jacinto, Ana M. Neves, Tatiana D. Vassilevskaia, Candido P. Ricardo, Claudina Rodrigues-Pousada

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Two different ubiquitin::79-amino-acid (aa) extension protein-encoding fusion genes were isolated from a Lupinus albus nuclear DNA library and sequenced. Both genes have 465-nucleotide open reading frames encoding a single ubiquitin (Ub) monomer fused in frame to a 79-aa extension (Ext) protein. The deduced aa sequences of the encoded Ub are identical to the aa sequences of Ub from other plants. The encoded Ext proteins are putative ribosomal proteins, highly basic, differing by 2 aa from each other, and have a high degree of similarity to Ext proteins from other plants.

Original languageEnglish
Pages (from-to)201-205
Number of pages5
JournalGene
Volume139
Issue number2
DOIs
Publication statusPublished - 25 Feb 1994

Keywords

  • codon usage
  • dicotyledons
  • Gene family
  • legumes
  • nuclear signal
  • ribosomal protein

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