TY - JOUR
T1 - Clonal expansion across the seas as seen through CPLP-TB database
T2 - a joint effort in cataloguing Mycobacterium tuberculosis genetic diversity in Portuguese-speaking countries
AU - Perdigao, Joao
AU - Silva, Carla
AU - Diniz, Jaciara
AU - Pereira, Catarina
AU - Machado, Diana
AU - Ramos, Jorge
AU - Silva, Hugo
AU - Abilleira, Fernanda
AU - Brum, Clarice
AU - Reis, Ana J.
AU - Macedo, Maira
AU - Scaini, Joao L.
AU - Silva, Ana B.
AU - Esteves, Leonardo
AU - Macedo, Rita Enrique
AU - Maltez, Fernando
AU - Clemente, Sofia
AU - Coelho, Elizabeth
AU - Viegas, Sofia
AU - Rabna, Paulo
AU - Rodrigues, Amabelia
AU - Taveira, Nuno
AU - Jordao, Luisa
AU - Kritski, Afranio
AU - Lapa e Silva, Jose R.
AU - Mokrousov, Igor
AU - Couvin, David
AU - Rastogi, Nalin
AU - Couto, Isabel
AU - Pain, Arnab
AU - McNerney, Ruth
AU - Clark, Taane G.
AU - von Groll, Andrea
AU - Dalla-Costa, Elis R.
AU - Rossetti, Maria Lucia
AU - Silva, Pedro E. A.
AU - Viveiros, Miguel
AU - Portugal, Isabel
PY - 2019/8
Y1 - 2019/8
N2 - Tuberculosis (TB) remains a major health problem within the Community of Portuguese Language Speaking Countries (CPLP). Despite the marked variation in TB incidence across its member-states and continued human migratory flux between countries, a considerable gap in the knowledge on the Mycobacterium tuberculosis population structure and strain circulation between the countries still exists. To address this, we have assembled and analysed the largest CPLP M. tuberculosis molecular and drug susceptibility dataset, comprised by a total of 1447 clinical isolates, including 423 multidrug-resistant isolates, from five CPLP countries. The data herein presented reinforces Latin American and Mediterranean (LAM) strains as the hallmark of M. tuberculosis populational structure in the CPLP coupled with country-specific differential prevalence of minor clades. Moreover, using high-resolution typing by 24-loci MIRU-VNTR, six cross-border genetic clusters were detected, thus supporting recent clonal expansion across the Lusophone space. To make this data available to the scientific community and public health authorities we developed CPLP-TB (available at http://cplp-tb.ff.ulisboa.pt), an online database coupled with web-based tools for exploratory data analysis. As a public health tool, it is expected to contribute to improved knowledge on the M. tuberculosis population structure and strain circulation within the CPLP, thus supporting the risk assessment of strain-specific trends.
AB - Tuberculosis (TB) remains a major health problem within the Community of Portuguese Language Speaking Countries (CPLP). Despite the marked variation in TB incidence across its member-states and continued human migratory flux between countries, a considerable gap in the knowledge on the Mycobacterium tuberculosis population structure and strain circulation between the countries still exists. To address this, we have assembled and analysed the largest CPLP M. tuberculosis molecular and drug susceptibility dataset, comprised by a total of 1447 clinical isolates, including 423 multidrug-resistant isolates, from five CPLP countries. The data herein presented reinforces Latin American and Mediterranean (LAM) strains as the hallmark of M. tuberculosis populational structure in the CPLP coupled with country-specific differential prevalence of minor clades. Moreover, using high-resolution typing by 24-loci MIRU-VNTR, six cross-border genetic clusters were detected, thus supporting recent clonal expansion across the Lusophone space. To make this data available to the scientific community and public health authorities we developed CPLP-TB (available at http://cplp-tb.ff.ulisboa.pt), an online database coupled with web-based tools for exploratory data analysis. As a public health tool, it is expected to contribute to improved knowledge on the M. tuberculosis population structure and strain circulation within the CPLP, thus supporting the risk assessment of strain-specific trends.
KW - MIRU-VNTR
KW - Spoligotyping
KW - Tuberculosis
KW - Drug resistance
KW - Migration
KW - Mycobacteria
KW - LAM
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85045092372&origin=resultslist&sort=plf-f&src=s&st1
UR - https://www.sciencedirect.com/science/article/pii/S1567134818301023?via%3Dihub
U2 - 10.1016/j.meegid.2018.03.011
DO - 10.1016/j.meegid.2018.03.011
M3 - Article
C2 - 29559379
SN - 1567-1348
VL - 72
SP - 44
EP - 58
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
ER -