Clonal diversity of ESBL-producing escherichia coli in pigs at slaughter level in Portugal

S. Ramos, N. Silva, D. Dias, M. Sousa, J. L. Capelo-Martinez, Francisco Brito, Manuela Caniça, Gilberto Igrejas, Patricia Poeta

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We aimed to determine the prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in fecal samples of healthy pigs, and to evaluate their clonality and associated resistance. Forty-nine percent of pigs sampled (n=35/71) in a slaughterhouse in Portugal revealed ESBL-producing E. coli isolates. Most isolates produced CTX-M-1 enzyme (71.4%; n=25/35), followed by CTX-M-9 (11.4%; n=4/35), CTX-M-14 (5.7%; n=2/35), SHV-12 (5.7%; n=2/35), and CTX-M-32 (5.7%; n=2/35). Ninety-four percent of the isolates presented a phenotype of multi-resistance. Most isolates belonged to phylogroups B1 (42.8%; n=15/35) and A (40%; n=14/35). Multilocus sequence typing (MLST) analysis revealed nine sequence types (STs) under six clonal complexes (CCs) and nine singletons, including overrepresentation of CC10 and three new STs (ST2524, ST2525, ST2528). We observed the frequent presence of CTX-M-producing E. coli in pigs at slaughter level, most of them belonging to CC10, commonly recovered from clinical samples. © Mary Ann Liebert, Inc.
Original languageEnglish
Pages (from-to)74-79
Number of pages6
JournalFoodborne Pathogens And Disease
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 2013

Keywords

  • antiinfective agent
  • beta lactamase CTX M
  • beta lactamase SHV
  • extended spectrum beta lactamase
  • beta lactamase
  • antibiotic resistance
  • antibiotic sensitivity
  • article
  • bacterium isolate
  • clonal variation
  • controlled study
  • Escherichia coli
  • feces analysis
  • gene cassette
  • gene insertion sequence
  • integron
  • multilocus sequence typing
  • nonhuman
  • phenotype
  • Portugal
  • priority journal
  • slaughterhouse
  • swine
  • animal
  • bacterium identification
  • disease carrier
  • DNA sequence
  • enzymology
  • Escherichia coli infection
  • feces
  • genetic variability
  • genetics
  • heterozygote
  • metabolism
  • microbial sensitivity test
  • microbiology
  • multidrug resistance
  • phylogeny
  • polymerase chain reaction
  • prevalence
  • swine disease
  • veterinary
  • Abattoirs
  • Animals
  • Bacterial Typing Techniques
  • beta-Lactamases
  • Carrier State
  • Disease Reservoirs
  • Drug Resistance, Multiple, Bacterial
  • Escherichia coli Infections
  • Feces
  • Genetic Variation
  • Microbial Sensitivity Tests
  • Multilocus Sequence Typing
  • Phenotype
  • Phylogeny
  • Polymerase Chain Reaction
  • Prevalence
  • Sequence Analysis, DNA
  • Swine
  • Swine Diseases

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