Clinical, laboratorial and immunological aspects of severe malaria in children from Guinea-Bissau

Domingos, Janine, Casimiro, Anaxore, Portugal-Calisto, Daniela, L Varandas, F Nogueira, MS Silva

Research output: Contribution to journalArticle

Abstract

Malaria is a parasitic disease of which Plasmodium falciparum causes the most severe form of the disease. The immune response against Plasmodium spp. is complex and remains unclear. The present report aimed to better understand the humoral immune response in severe malaria and analyse new immunodominant antigen candidates as possible serological marker in severe malaria in children. This study included children aged 0–16 years from Guinea-Bissau with clinical signs of severe malaria. Serological and immunochemical characterisation of different anti-P. falciparum antibodies were made by ELISA and immunoblotting using a crude protein extract of P. falciparum. Sera from 12 children with severe malaria were analysed. Nine samples were positive for total anti-P. falciparum antibodies, seven for IgM and eight for total IgG anti-P. falciparum. There was a predominance of IgG1 response, suggesting a cytophilic action in severe malaria and a major role of IgG1 over other immunoglobulins. The antigenic profile of P. falciparum showed a consistent immunoblotting pattern of approximately 180 kDa, 100 kDa and around 50–40 kDa. The serological reactivity found in protein bands makes them as immunodominant antigens and promising candidates for serological markers in the context of severe malaria.
Original languageEnglish
Pages (from-to)46-51
Number of pages6
JournalActa Tropica
VolumeVol. 185
DOIs
Publication statusPublished - 21 Apr 2018

Fingerprint

Guinea-Bissau
Malaria
Plasmodium falciparum
Immunodominant Epitopes
Immunoblotting
Immunoglobulin G
Parasitic Diseases
Plasmodium
Antibodies
Humoral Immunity
Complex Mixtures
Immunoglobulin M
Immunoglobulins
Proteins
Enzyme-Linked Immunosorbent Assay

Keywords

  • Humoral immunity
  • Malaria antigens
  • P. falciparum antibodies
  • Plasmodium falciparum
  • Severe malaria

Cite this

Janine, Domingos, ; Anaxore, Casimiro, ; Daniela, Portugal-Calisto, ; Varandas, L ; Nogueira, F ; Silva, MS. / Clinical, laboratorial and immunological aspects of severe malaria in children from Guinea-Bissau. In: Acta Tropica. 2018 ; Vol. Vol. 185. pp. 46-51.
@article{a1b17d3b213545539506f475d61f7289,
title = "Clinical, laboratorial and immunological aspects of severe malaria in children from Guinea-Bissau",
abstract = "Malaria is a parasitic disease of which Plasmodium falciparum causes the most severe form of the disease. The immune response against Plasmodium spp. is complex and remains unclear. The present report aimed to better understand the humoral immune response in severe malaria and analyse new immunodominant antigen candidates as possible serological marker in severe malaria in children. This study included children aged 0–16 years from Guinea-Bissau with clinical signs of severe malaria. Serological and immunochemical characterisation of different anti-P. falciparum antibodies were made by ELISA and immunoblotting using a crude protein extract of P. falciparum. Sera from 12 children with severe malaria were analysed. Nine samples were positive for total anti-P. falciparum antibodies, seven for IgM and eight for total IgG anti-P. falciparum. There was a predominance of IgG1 response, suggesting a cytophilic action in severe malaria and a major role of IgG1 over other immunoglobulins. The antigenic profile of P. falciparum showed a consistent immunoblotting pattern of approximately 180 kDa, 100 kDa and around 50–40 kDa. The serological reactivity found in protein bands makes them as immunodominant antigens and promising candidates for serological markers in the context of severe malaria.",
keywords = "Humoral immunity, Malaria antigens, P. falciparum antibodies, Plasmodium falciparum, Severe malaria",
author = "Domingos, Janine and Casimiro, Anaxore and Portugal-Calisto, Daniela and L Varandas and F Nogueira and MS Silva",
year = "2018",
month = "4",
day = "21",
doi = "10.1016/j.actatropica.2018.04.020",
language = "English",
volume = "Vol. 185",
pages = "46--51",
journal = "Acta Tropica",
issn = "0001-706X",
publisher = "Elsevier Science B.V., Amsterdam.",

}

Clinical, laboratorial and immunological aspects of severe malaria in children from Guinea-Bissau. / Janine, Domingos, ; Anaxore, Casimiro, ; Daniela, Portugal-Calisto,; Varandas, L; Nogueira, F; Silva, MS.

In: Acta Tropica, Vol. Vol. 185, 21.04.2018, p. 46-51.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Clinical, laboratorial and immunological aspects of severe malaria in children from Guinea-Bissau

AU - Janine, Domingos,

AU - Anaxore, Casimiro,

AU - Daniela, Portugal-Calisto,

AU - Varandas, L

AU - Nogueira, F

AU - Silva, MS

PY - 2018/4/21

Y1 - 2018/4/21

N2 - Malaria is a parasitic disease of which Plasmodium falciparum causes the most severe form of the disease. The immune response against Plasmodium spp. is complex and remains unclear. The present report aimed to better understand the humoral immune response in severe malaria and analyse new immunodominant antigen candidates as possible serological marker in severe malaria in children. This study included children aged 0–16 years from Guinea-Bissau with clinical signs of severe malaria. Serological and immunochemical characterisation of different anti-P. falciparum antibodies were made by ELISA and immunoblotting using a crude protein extract of P. falciparum. Sera from 12 children with severe malaria were analysed. Nine samples were positive for total anti-P. falciparum antibodies, seven for IgM and eight for total IgG anti-P. falciparum. There was a predominance of IgG1 response, suggesting a cytophilic action in severe malaria and a major role of IgG1 over other immunoglobulins. The antigenic profile of P. falciparum showed a consistent immunoblotting pattern of approximately 180 kDa, 100 kDa and around 50–40 kDa. The serological reactivity found in protein bands makes them as immunodominant antigens and promising candidates for serological markers in the context of severe malaria.

AB - Malaria is a parasitic disease of which Plasmodium falciparum causes the most severe form of the disease. The immune response against Plasmodium spp. is complex and remains unclear. The present report aimed to better understand the humoral immune response in severe malaria and analyse new immunodominant antigen candidates as possible serological marker in severe malaria in children. This study included children aged 0–16 years from Guinea-Bissau with clinical signs of severe malaria. Serological and immunochemical characterisation of different anti-P. falciparum antibodies were made by ELISA and immunoblotting using a crude protein extract of P. falciparum. Sera from 12 children with severe malaria were analysed. Nine samples were positive for total anti-P. falciparum antibodies, seven for IgM and eight for total IgG anti-P. falciparum. There was a predominance of IgG1 response, suggesting a cytophilic action in severe malaria and a major role of IgG1 over other immunoglobulins. The antigenic profile of P. falciparum showed a consistent immunoblotting pattern of approximately 180 kDa, 100 kDa and around 50–40 kDa. The serological reactivity found in protein bands makes them as immunodominant antigens and promising candidates for serological markers in the context of severe malaria.

KW - Humoral immunity

KW - Malaria antigens

KW - P. falciparum antibodies

KW - Plasmodium falciparum

KW - Severe malaria

U2 - 10.1016/j.actatropica.2018.04.020

DO - 10.1016/j.actatropica.2018.04.020

M3 - Article

VL - Vol. 185

SP - 46

EP - 51

JO - Acta Tropica

JF - Acta Tropica

SN - 0001-706X

ER -